氟化钠及其拮抗剂硫酸锌对成骨细胞的影响

Effect of sodium fluoride and its antagonist zine sulfate on rat osteoblasts

目的 观察不同剂量氟化钠 ( Na F)对成骨细胞的影响及硫酸锌 ( Zn SO4 )的拮抗作用 ,为临床治疗提供实验依据。方法 取新生 2 4 h SD鼠头盖骨分离成骨细胞 ,在不同浓度的 Na F和 /或 Zn SO4( 10 -5mol/L)中培养。经 MTT法测定细胞增殖 ,细胞分化由碱性磷酸酶 ( AL P)和骨钙素水平测定得到。结果 Na F对成骨细胞增殖及分化呈双向调节 ,主要表现为大剂量抑制 ,小剂量促进。当 Na F浓度为10 -6mol/L及 10 -5mol/L时 ,细胞增殖及碱性磷酸酶活性 ( AL P)均增加 ( P<0 .0 5)。但当 Na F浓度增加至 10 -4 mol/L及 5× 10 -4 mol/L时 ,其对细胞增殖及 ALP活性呈现抑制作用。然而 ,不同剂量的 Na F对骨钙素均表现为促进其分泌 ( P<0 .0 5)。Zn SO4 与低浓度的 Na F( 10 -6mol/L、10 -5mol/L)有协同作用 ,而对高浓度的 Na F( 10 -4 mol/L、5× 10 -4 mol/L )拮抗 ,使 Na F对细胞增殖率及 ALP活性的抑制作用减弱 ,并降低高浓度 Na F所刺激的骨钙素分泌。结论 Na F对离体成骨细胞的增殖呈双向调节 ,而对细胞分化的影响呈多样性 ,早期表现为剂量依赖性双向调节 ,后期仅呈现单向刺激。 10 -5mol/L Zn SO4 拮抗大剂量 Na F,对低浓度 Na

Objective\ To assess the effect of sodium fluoride(NaF)on rat osteoblasts and the antagonism of zinc sulfate(ZnSO 4)on NaF.Methods\ Osteoblasts obtained from the calvariae of newborn rats were cultured in medium with different concentrations of NaF and /or ZnSO 4.Cell proliferation was detected by MTT method and differentiation was determined by measuring alkaline phosphatase(ALP) activity and osteocalcin secretion.Results NaF increased cell proliferation and differentiation in a double regulation manner,i.e.low doses of NaF stimulated both proliferation and ALP activity of osteoblasts,while high doses suppressed them. However,all doses of NaF we used increased osteocalcin production.ZnSO 4 was synergistic with low doses of NaF and antagonistic to high doses of NaF by restoring partly the suppressive effect of NaF on cell proliferation and ALP activity,as well decreasing the enhancement of osteocalcin secretion by NaF. Conclusion NaF acts on osteoblast proliferation in a double regulated manner and possesses multiple effects on cell differentiation.10 -5 M ZnSO 4 antagonizes high doses of NaF,but does not antagonizes low doses of NaF.