EZH2和FOXC1在基底细胞样型乳腺癌中的表达及其意义

Expression and Clinical Pathological Features of EZH2 and FOXC1 in Basal-like Breast Cancer

目的检测基底细胞样型乳腺癌中EZH2、FOXC1的表达,分析其相关性及与临床病理特征和预后的关系。方法采用免疫组化方法检测45例基底细胞样乳腺癌、75例其它类型乳腺浸润性导管癌及15例乳腺腺病组织中EZH2、FOXC1的表达。结果 EZH2、FOXC1在基底细胞样乳腺癌表达率明显高于其它类型浸润性乳腺癌及乳腺腺病组织(P<0.05);EZH2异常表达与FOXC1高表达呈正相关(r=0.433,P<0.05);EZH2在不同组织分化程度及有无淋巴结转移的基底细胞样乳腺癌中的表达差异有统计学意义(P<0.05),但在不同民族、年龄和肿瘤大小之间差异无统计学意义;FOXC1在不同组织学分级的基底细胞样乳腺癌中的表达差异有统计学意义(P<0.05),在不同民族、年龄、肿瘤大小和有无淋巴结转移之间差异无统计学意义;3年无瘤生存率,EZH2、FOXC1的阳性表达差异均有统计学意义。结论 EZH2、FOXC1是基底细胞样乳腺癌侵袭转移和预后重要的分子标志。

Objective To investigate the expression of tumor suppressor gene EZH2 and FOXC1 in female basal - like breast cancer , and to explore their relationship to clinical features and prognosis. Methods Tis-sue microarray techniques and Immunohistochemistry ElivisionTMsuper method were used to detect the expression of EZH2 and FOXC1 in 45 cases with basal - like breast cancer and 75 cases with other type breast cancer and 15 cases with normal breast tissue. Results High expression of EZH2 and FOXC1 was found in basal -like breast cancer, while theirs expression was significantly lower in other type breast cancer and normalbreast tissue samples （P 〈 0.05）. Furthermore, the aberrant expression of EZH2 was correlated with the over- expression of FOXC1 in basal - like breast cancer（r = 0. 433,P 〈 0.05）. EZH2 expression was significantlyassociated with the differentiations and lymph node metastasis, resepectively （ P 〈 0.05 ）. But in different eth- nicity, age and tumor size EZH2 expression had no statistical significance （ P 〉 0.05 ）. While FOXC1 expres-sion was only significantly associated with the differentiation, resepectively （ P 〈 0.05 ）. And in different ethnicity, age, tumor size and lymph node metastasis FOXC1 expression had no statistical significance （P 〉0.05 ）. The five - year survival rate was significantly higher in patients with negativeEZH2 and FOXC1 expression than that in patients with positive EZH2 and FOXC1 expression.Conclusion EZH2 and FOXC1 has been identified as a molecular marker that distinguishes indolent basal - like breast cancer from those at risk of lethal progression, and as a possible target for preventative interven-tion,while FOXC1 is an important factor excluding of lymph node metastasis. FOXC1 may serve as a new biomarkers for diagnosing and predicting the biological behavior and targeting treatment for patients with basal -like breast cancers.