P^(16)抑癌基因与喉癌颈淋巴结转移和恶性度关系的探讨

Relationship of expression of tumor suppressor gene P^(16) and cervical lymph node metastasis, malignity on laryngeal carcinoma

目的 :为了探讨 P1 6蛋白表达与喉癌颈淋巴结转移及与癌组织恶性度的关系。方法 :用免疫组化 SP法对 82例声门上型喉癌的原发灶、癌旁组织及颈淋巴结进行了 P1 6蛋白表达的检测。结果 :喉癌原发灶中 P1 6蛋白表达阳性率 5 4.9% ;其中无颈淋巴结转移组阳性率为 6 8.3% ;有颈淋巴结转移组的阳性率 41 .5 % ,差异有显著性意义 (P <0 .0 5 )。低度恶性组原发灶中 P1 6蛋白表达阳性率 6 2 .2 % ;高度恶性组原发灶中 P1 6蛋白表达阳性率 41 .7% ,差异显著 (P <0 .0 5 )。高度恶性及原发灶 P1 6蛋白表达阴性组颈淋巴结转移率 81 .0 % ;低度恶性原发灶 P1 6蛋白表达阳性组颈淋巴结转移率 33.3% ,转移率间也有显著性差异 (P <0 .0 0 1 )。P1 6基因及其蛋白产物与喉癌的恶性度及颈淋巴结转移有相关倾向。结论 :临床检测 P1

Objective:To study the relationship of tumor suppressor gene P 16 and cervical lymph node metastasis and malignity of laryngeal carcinoma.Methods:The expression of P 16 protein in primary site,near primary site and cervical lymph node were tested by immunohistochemical SP method in 82 patients with supraglottis carcinoma.Results:(1) The positive rate of P 16 protein expression was 54.9%(45/82) in primary site of all 82 patients with laryngeal carcinoma.(2) Comparison between the group of without lymph node metastasis and group with lymph node metastasis,the positive rates of the expression of P 16 protein,which were significantly different (P<0 05),there were 68.3%(28/41) and 41.5%(17/41) respectively.(3) Cervical lymph node metastasis rate was 81.0%(17/21) in the group with severe malignity and negative expression of P 16 protein,and 33.3%(10/30) in the group with mild malignity and positive expression of P 16 protein (P<0 001).It showed that the expression of tumor suppressor gene P 16 was closely related to cervical lymph node metastasis of laryngeal carcinoma.Conclusion:The clinical examination of the expression of P 16 protein should be valuble for the diagnosis of malignity and cervical lymph node metastasis in patients with supraglottis carcinoma. [Chinese Arch Otolaryngol Head Neck Surg,2000;7(4):229~232 From the Department of Otolaryngology,First Clinical College of Chinese Medical University,Shenyang,110001 (Dr.Yu Gang)]