ELISA和TLC检测饲料中AFB_1含量的比较研究

Comparison on AFB_1 Content in Feed Determined by ELISA and TLC Methods

[目的]研究酶联免疫吸附法和薄层色谱法的特点,比较2种方法检测黄曲霉毒素B1含量的优缺点。[方法]酶联免疫吸附法采用黄曲霉毒素B1酶联免疫定量测试盒对饲料中黄曲霉毒素B1的含量进行测定;薄层色谱法应用薄层荧光扫描测定黄曲霉毒素B1的含量,并用薄层色谱进行确证试验。[结果]酶联免疫吸附法测定黄曲霉毒素B1含量的标准曲线r值为0.9993～0.9999,变异系数为0.3%～0.6%,回收率在94.0%～105.0%之间。薄层色谱为半定量色谱,但该试验利用荧光扫描法测定黄曲霉毒素B1的含量,标准曲线r值为0.9981,回收率在84.60%～90.08%之间。[结论]2种方法相比较,薄层色谱法是传统经典的方法,具有抗干扰能力强、离线检测可反复测试的优点,但TLC法前处理较为麻烦,精密度、回收率均没有酶联免疫吸附法高;ELISA法是目前国际检测AFB1的先进方法,具有灵敏度高、干扰少、简便快速、操作安全等优点。

[Objective] The aim was to study the characteristics of ELISA method and thin layer chromatography（TLC） and compare advantages and disadvantages of aflatoxin B1 content determined by the two ways.[Method] The content of aflatoxin B1 was determined by using aflatoxin B1 enzyme league immune in ELISA method,while it was determined by using thin fluorescence scanning method in TLC method.[Result] The standard curve r value of aflatoxin B1 content determined by ELISA method was 0.999 3-0.999 9;the variation coefficient was 0.3%-0.6%,and the recovery was between 94.0% and 105.0%.TLC was half quantitative chromatography,but the standard curve r value of aflatoxin B1 content determined by using fluorescence scanning method was 0.998 1,and the recovery was between 84.60% and 90.08%.[Conclusion] Compared the two methods,TLC is traditional classical method,has advantages of strong ability anti-interference dream,offline testing and repeatedly test,but it also has more troubles before treatment,and has lower precision and recovery than ELISA method;ELISA is the international advanced method to detect AFB1,and has advantages of high sensitivity,less disturbance,simple,quick and safe operation.