HCV-F蛋白的原核表达以及对Th1/Th2型细胞因子的影响

Expression of escherichia coli derived HCV 1b F protein and its modulation of Th1/Th2 balance in PBMC of patients with hepatitis C without treatment

目的探讨丙型肝炎病毒(hepatitis C virus,HCV)1b型F蛋白对Th1/Th2型细胞因子的影响。方法收集慢性HCV患者(chronic hepatitis patient,CHP)和HCV肝癌患者(hepatocellular carcinoma patient,HCCP)的血样,检测F抗体(F antibody,F-Ab)的阳性率并且分成4组,F-Ab(+)CHP、F-Ab(-)CHP、F-Ab(+)HCCP、F-Ab(-)HCCP;分离患者外周血单核细胞(peripheral blood mononuclear cell,PBMC),用F蛋白刺激,72 h后用酶联免疫吸附试验(enzyme-linked immunosorbent assay,ELISA)法检测细胞上清干扰素γ(interferon-γ,IFN-γ)和白介素5(in-terluekin-5,IL-5)水平。结果 F-Ab(-)CHP组IFN-γ高于F-Ab(+)CHP组(P=0.020);F-Ab(-)HCCP组IFN-γ高于F-Ab(+)HCCP组(P=0.019),F-Ab(+)HCCP组IL-5的水平高于F-Ab(-)HCCP组(P=0.032);F-Ab(+)CHP组IL-5的水平高于F-Ab(-)CHP组(P=0.041)。结论在体外培养的PBMC中,F蛋白有上调IL-5水平和下调IFN-γ水平的作用,提示可能与肝炎慢性化和肝癌的发生机制有关。

Objective To investigate the regulatory effect of hepatitis C virus lb subtype F protein on Th1/Th2 cytokine . Methods Blood samples were collected from hepatitis C virus （HCV） related chronic hepatitis patients （CHP） and HCV related hepatocellular carcinoma patients （HCCP）. F protein was used to detect F antibodies in the serum to di- vide samples into four subject groups including F-Ab（ ＋ ）CHP, F-Ab（ - ）CHP, F-Ab（ ＋ ）HCCP and F-Ab（ - ） HCCP. PBMCs were isolated from these four groups and then treated with F protein. After 72 hours, enzyme-linked immunosorbent assay（ELISA） was used to detect the concentration of IFN--,/ and IL-5. Results IFN-γ levels of F-Ab （ - ） CHP were higher than those of F-Ab（ ＋ ） CHP（P = 0. 020） and F-Ab（ - ） HCCP higher than those of F-Ab（ ＋ ） HCCP（P = 0. 019）. IL-5 levels of F-Ab（ ＋ ） CHP were higher than those of F-Ab（ - ） CHP（PL = 0. 032,P2 = 0. 041 ）. Conclusions F pro- tein could trigger the expression of IL-5 and inhibit the expression of IFN-γ in vitro and therefore might play a role in the HCV pathogenesis and might be a useful predictor for chronicity.