颈椎舒颗粒对椎间盘退变大鼠模型AQP1的影响

The Effect of Jingzhuishu Granule on AQP1 in Rat Degeneration Cervical Intervertebral Disc

目的:探讨颈椎舒颗粒治疗颈椎病作用的机制。方法:以动静力失衡方法建立颈椎间盘退变模型,测定椎间盘含水量了解颈椎间盘退变情况,用免疫印迹法检测大鼠椎间盘中AQP1含量,观察模型大鼠给药4周、8周后的变化。结果:其中用药4周后,与模型组相比,各组组均能增加椎间盘的含水量,差异有显著性意义(P<0.05)。各药物干预组,以高剂量组疗效最佳,高剂量组含水量最高,模型组最低。用药8周后,与模型组相比,各组组均能增加椎间盘的含水量,差异有显著性意义(P<0.05)。各药物干预组,以高剂量组疗效最佳,高剂量组含水量最高,模型组最低。用药4周后,与模型组相比,各组均能增加颈椎间盘AQP1的含量,差异有显著性意义(P<0.05)。各药物干预组,以高剂量组疗效最佳,高剂量组颈椎间盘AQP1的含量最高,模型组最低。用药8周后,与模型组相比,各组组均能增加颈椎间盘AQP1的含量,差异有显著性意义(P<0.05)。各药物干预组,以高剂量组疗效最佳,高剂量组AQP1最高,模型组最低,并且随着治疗时间的延长,效果有增加的趋势。结论:颈椎舒具有改善颈椎间盘退变的作用,对水通道蛋白1的调节作用是其疗效机制之一。

Objective： Modified jingzhuishu Granule observed clinical efficacy in the treatment of degeneration cervical intervertebral disc.Methods： Cervical disc degeneration model： static and dynamic force imbalance,and determination of disc water content for cervical disc degeneration,detected by Western blotting of AQP1 in the rat intervertebral disc content,the observation model in rats administered 4 weeks,8 weeks afterchanged.Results： After 4 weeks,compared with model group,each group can increase the water content of the disc,the difference was significant（P0.05）.Each drug in the intervention group,the best efficacy to the high dose group,the highest water content of the high dose group,model group had the lowest.After 8 weeks,compared with model group,each group can increase the water content of the disc,the difference was significant（P0.05）.Each drug in the intervention group,the best efficacy to the high dose group,the highest water content of the high dose group,model group had the lowest.After four weeks,compared with model group,the group could increase the content of the cervical intervertebral disc of AQP1,a significant difference（P0.05）.The drug intervention group,the best efficacy to the high dose group,the highest content of the cervical intervertebral disc of high-dose group of AQP1,the model group had the lowest.After 8 weeks,compared with model group,each group can increase the content of AQP1 in cervical disc,the difference was significant（P0.05）.Each drug in the intervention group,the best efficacy to the high dose group,AQP1 highest of the high dose group,model group had the lowest.With the extension of treatment time,the effect is on the rise.Conclusion： cervical Shu improve cervical disc degeneration,and regulation of aquaporin 1 is one of the mechanisms of its efficacy.