摘要
目的:以穿心莲药材中穿心莲内酯与脱水穿心莲内酯的含量为指标,考察广西GAP基地与普通产地穿心莲药材的优劣;方法:采用HPLC法,色谱柱为Diamonsil-ODS C18(250mm×4.6mm,5μ);流动相为甲醇-水(65:35);流速为0.8ml/min;检测波长为225nm(0~15min)和254nm(15~25min),柱温:25℃;结果:GAP基地所产穿心莲药材普遍优于普通产地的药材;结论:实施药材GAP规范化种植是保证中药药源质量的有效途径。
Objective To investigate the content of andrographolide and dehydro - andrographolide in Andrographis paniculata (Burm. f. ) Nees from GAP bases and ordinary sources, then judged the better ones; Methods Diamonsil -ODS C is (5μ 250 × 4. 6mm) columu was adopted, the mobile phase consisted of methanol -water (65: 35) eluted at the flow rate of 0. 8ml/min, the de- tection wave length were 225nm (0 - 15rain) and 254nm ( 15 -25min) ; the column temperature was 25μ ; Results The products from GAP bases were better than those from ordinary sources; Conclusion: GAP implantation was a positive way to insure that the qual- ities of TCM were ascendencily.
出处
《中国民族民间医药》
2013年第5期17-18,20,共3页
Chinese Journal of Ethnomedicine and Ethnopharmacy
关键词
穿心莲
穿心莲内酯
脱水穿心莲内酯
HPLC
GAP基地
Andrographis paniculata ( Burm. f. ) Nees
andrographolide
dehydro - andrographolide
HPLC
GAP bases