三种对虾病毒在浙江省凡纳滨对虾中的流行性调查研究

Prevalence of Three Viruses in Pacific White Leg Shrimp Litopenaeus vannamei in Zhejiang Province

对虾白斑综合症病毒、桃拉综合症病毒及传染性皮下与造血组织坏死病病毒是目前威胁对虾养殖业较为严重的3种病毒。分别从浙江省6个凡纳滨对虾的主养殖区随机采样对虾699尾,通过PCR检测对虾的病毒携带情况。检测结果显示,6个养殖地区传染性皮下造血组织坏死病病毒检测结果均呈阳性,总阳性率达100%;萧山、舟山、象山养殖场的对虾白斑综合症病毒检测结果有阳性,总阳性率为5.58%;6个养殖场均未检测出桃拉综合症病毒。表明浙江省凡纳滨对虾养殖区域的桃拉综合症病毒得到了有效控制,传染性皮下造血组织坏死病病毒和对虾白班综合症病毒有较高的感染率,而且存在混合感染的情况。

White spot syndrome virus （WSSV）, Taura syndrome virus （TSV） and infectious hypodermal and hematopoietic necrosis virus （IHHNV） are the three major pathogenic viruses responsible for pandemics diseases threatening Pacific white leg shrimp（Litopenaeus vannamei） farming industry. A total of 669 shrimp samples were randomly collected from 6 major shrimp farming areas in Zhejiang province, and observed on the prevalence of these viruses by PCR analysis. Results showed that the positive reactions for IHHNV were observed in all the 6 locations, with a total positive rate of 100% and the positive reactions for WSSV were found in the samples collected from Xiaoshan, Zhoushan and Xiangshan, with a total positive rate of 5.58%. The co-infection with IHHNV and WSSV was detected in the shrimp samples. No shrimp sample was found to exhibit the evidence of infection with TSV, indicating that the prevalence of TSV was found to be decreased significantly in Zhejiang province.