双酚A对小鼠心脏损伤及其机制初步探讨

Study on the Heart Injury by Bisphenol-A and its Mechanisms in Mice

目的初步探讨双酚A对小鼠心脏组织的损伤作用。方法 50只健康昆明(KM)小鼠,按体重随机分为5组,每组10只,雌雄各半。剂量组分别给予2.4、24、240、480 mg/kg双酚A灌胃染毒,对照组给予玉米油灌胃,每天1次,共28 d。试验中计算心脏脏器系数,荧光分光光度法测定心肌组织中Ca2+-ATP酶的活性,通过HE染色观察心肌组织病理变化。结果与对照组比较,染毒组小鼠Ca2+-ATP酶活性下降(P<0.05),心脏脏器系数差异无统计学意义(P>0.05)。病理组织学检查可见心脏组织出现不同程度的改变,且随着剂量增加病理改变程度越显著。主要表现为心肌纤维排列疏松,间隙增宽,间质水肿,大量炎症细胞浸润,甚至心肌坏死。雌雄组之间无差异。结论双酚A对小鼠心脏组织可造成一定的损伤,其机制可能与双酚A导致小鼠心肌组织炎症与Ca2+-ATP酶活性下降有关。

Objective To preliminarily explore the heart injury by bisphenol-A（BPA） in mice.Methods Fifty healthy Kunming mice were randomly assigned into 5 groups with 10 mice,5 males and 5 females,for each group.The mice in the BPA-exposed groups were gavaged with 2.4,24,240 or 480mg/kg·bw BPA daily for 28 days,and the mice in the control group were gavaged with corn oil.At the end of the experiment,the organ coefficient of heart was calculated.The Ca2＋-ATPase activity in the cardiac tissue was determined.And the pathological changes in the cardiac tissue were observed.Results Compared with the control group,the Ca2＋-ATPase activity in the cardiac tissue of the mice in the BPA-exposed groups was significantly decreased（P0.05）.The organ coefficients of heart were not statistically different among various groups（P0.05）.Pathological changes were present in the myocardial tissue of the mice exposed to BPA,and the changes were more apparent with the increase of the exposed BPA doses.The main pathological changes included rarefactions and diastema widening of cardiac myofibril arrangements,interstitial edema,a large number of inflammatory cells infiltration and even myocardial necrosis.The Ca2＋-ATPase activity and the pathological changes were not statistically different between the male and female mice.Conclusions Bisphenol A can induce heart injury in mice.The mechanism may involve the inducing of inflammation and the decrease of Ca2＋-ATPase activity in myocardial tissue.