摘要
研究中药材百部根石油醚提取物中最佳流分9对朱砂叶螨生物活性的作用机理。运用玻片浸渍法测定了流分9对朱砂叶螨成螨的室内毒力,并运用生化方法对乙酰胆碱酯酶、单胺氧化酶、Ca2+-ATP酶、超氧化物歧化酶(SOD)活性的影响进行了测定。表明百部流分9对朱砂叶螨成螨有很强的生物活性,对成螨的LC50是0.6339mg/mL。百部流分9处理朱砂叶螨后,螨体内超氧化物歧化酶(SOD)被激活,而单胺氧化酶、Ca2+-ATP酶、乙酰胆碱酯酶均受到明显的抑制,这一系列变化可能会引起螨类神经系统传递的阻断,因此导致害螨的死亡。由此可见百部可以有效杀死朱砂叶螨,作为新型植物源农药具有很大的开发潜力。
The aim of this study was to determine the acaricidal mechanism of fraction 9 of radix stemonae extracts against T. cinnabarinus. The glass slide-dipping method was used to determine acaricidal activity of the fraction 9 of radix stemonae extracts against T. einnabarinus. The effects of the fraction 9 of radix stemonae extracts on four important enzyme activities including ACHE, Ca^（2＋）-ATPase, SOD and MAO were determined by biochemistry method. The studies showed that the fraction 9 of radix stemonae extracts had high toxicity to T. cinnabarinus with a LC50 value of 0.6339 mg/mL. After exposure to fraction 9 of radix stemonae extracts, the activity of SOD was activated, while the activities of ACHE, Ca^（2＋）-ATPase, and MAO in T. cinnabarinus were inhibited at different degrees. The main enzyme activities of the mites were changed by fraction 9 of radix stemonae extracts, which might kill the mites. The results showed that radix stemonae extracts had high acaricidal activities to T. cinnabarinus, and radix stemonae could be a novel resource of botanical acaricides.
出处
《农学学报》
2013年第3期13-17,38,共6页
Journal of Agriculture
基金
教育部重点项目"以朱砂叶螨乙酰胆碱酯酶为靶标筛选新型植物源杀螨剂模型的构建"(212001)
北京市教育委员会科技计划面上项目"朱砂叶螨杀螨剂神经靶标乙酰胆碱酯酶基因的克隆与表达特征分析"(KM201210020001)
北京市教委平台建设项目(PXM2012_014207_000014
PXM2012_014207_000028
PXM2012_014207_000016)
北京市教委科技成果转化与产业化(PXM2011_014207_000026)
