一组混合菌群还原硫酸盐的特性

Study of the Physiology on Sulfate Reducing Bacteria

为了利用硫酸盐还原法消除硫酸盐和重金属离子对环境的污染,从凤阳城区排水沟中富集和驯化获得硫酸盐还原菌菌群,研究了硫酸盐还原菌群对含硫氧酸盐的还原,以及温度、pH、盐度、氧气和Fe2+等因素对菌群还原硫酸盐能力的影响。结果表明,该菌群可以利用乳酸钠还原硫代硫酸盐、硫酸盐,不能还原亚硫酸盐;最适合的生长温度为36℃,pH7.5,可在3%的氯化钠浓度下生长;在兼性厌氧条件下对硫酸盐还原能力最强;硫酸盐还原率随着Fe2+浓度的增加而增大。菌群接种量为10%,FeSO4初始浓度为2000mg/L,培养30h后硫酸盐还原率达到95%以上。

In order to utilize sulfate-reducing method on the treatment of heavy metal wastewater and organic wastewater containing sulfate,sulfate reducing bacteria （SRB） were enriched from the long-term polluted sewer in Fengyang,and their removing sulfate abilities were studied in the different conditions,such as：sulfur source,temperature,pH value,salinity,oxygen and the concentration of Fe 2 ＋.The results showed that,CH 3 CHOHCOONa could be used as a carbon source to reduce sulfate and thiosulfate,and sulfite could not be reduced.The optimum conditions for SRB growth were found to be at 36℃,pH 7.5.SRB were able to grow in the NaCl concentration of 3%,meanwhile,their removing sulfate abilities was the best in facultative anaerobic;the rate of sulfate reducing was increasing with the increased Fe 2＋ concentration.The rate of sulfate reducing would reach above 95% after thirty hours when SRB were inoculated by 10% and the concentration of FeSO 4 was 2000 mg/L.