贻贝棘尾虫类催乳素的免疫细胞化学研究

An Immunocytochemical Study of Prolactin-like in A Ciliated Protozoan Stylonychia mytilys

为进一步证实原生动物体内是否存在着类似高等动物的激素类物质。选取单细胞原生动物下毛目散毛亚目的纤毛虫贻贝棘尾虫(Stylonychia mytilus)为研究对象,使用兔抗人催乳素抗体,运用B-SA(Biotin-streptavidin amplified)免疫细胞化学染色技术,在光学显微镜下观察催乳素(Prolactin,PRL)在贻贝棘尾虫体内的分布状况。结果显示:贻贝棘尾虫的表膜、棘毛、口围带、波动膜染色浓重,分布密集;细胞质部分染色较浅,分布稀疏;其大核呈阴性反应。暗示在贻贝棘尾虫体内可能存在着一种似脊椎动物体内的类PRL物质。这与一些类神经肽物质在贻贝棘尾虫体内的分布基本相同,也就是光镜下多种一抗的结合位点基本一致,作者认为这是原生动物中对生命活动起调控作用的物质处于原始初级形态,尚未进行分化或分化的不彻底,专一性不强的表现,故多种抗体能与其结合发生阳性反应。

To further confirm whether similar hormone in the high animal could exist in the protozoa, the research was conducted with the distribution of Rabbit Anti-human Prolactin using the immunocytochemical staining technique in Stylonychia mytilys under optical microscope. The results indicated that pellicle, cirri, adoral zone membranelle （AZM） and undulating membrane （UM） of Stylonychia mytilys were heavily stained and thickly distributed, but cytoplasm were lightly stained and densely distributed. Macronuclei reactions were negative. This was in accordance with the localization of neuropeptide substances in Stylonychia mytilys. That was generally consistent with combining sites of different first antibodies. Because they had not still differentiated or differentiated incompletely, the substances regulating vital movement were still in primary form so that they could took on positive reaction with kinds of antibodies.