丙氨瑞林治疗后子宫内膜癌细胞有丝分裂指数、细胞周期及超微结构的变化

Mitotic index, cell cycle and ultranstructure changes of endometrial carcinoma after treatment of Alarelin

目的：研究丙氨瑞林对子宫内膜癌的作用及机理。方法：对诊刮确诊为子宫内膜癌的１７例患者，肌肉注射丙氨瑞林２００μｇ，每天１次×７ｄ。用药结束后３ｄ行手术治疗。检测用药前、后癌细胞有丝分裂指数（ｍｉｔｏｔｉｃ ｉｎｄｅｘ，ＭＩ）；并运用流式细胞术及透射电镜观察子宫内膜样腺癌８例用药前后癌细胞的细胞周期变化及超微结构变化。结果：内膜样腺癌１３例用药后ＭＩ下降（Ｐ＜０．０１），８例用药后癌细胞Ｓ期下降（Ｐ＜０．０５）；癌细胞中线粒体、粗面内质网等减少，核内常染色质减少，异染色质增多，核仁固缩或呈圈状。结论：丙氨瑞林短期治疗子宫内膜癌，尤其是子宫内膜样癌，可抑制癌细胞的合成代谢与分裂。

Objective To study the action and mechanism of Alarelin on endometrial carcinoma Methods: Seventeen patients with endometrial cancer were given Alarelin 200μg im qd for seven days and three days later an operation was given .Before and after the Alarelin therapy, the neoplasm samples were taken for HE stains and the mitotic index(MI) was observe d. Flow cytometry and transmission electron microscopy were used to study the change of cell cycle and ultrastructure in eight endometrioid carcinoma patients. Results:MI in 13 patients with endometrioid cancer were decreased sign ificantly(P<0.01) after Alarelin th erapy In 8 endometrioid cancer patients, Sphase rate declined(P<0. 05) Rough -surfaced endoplasmic reticules and mito chondrion were fewer, euchromatin decreased and heterochromatin increased. Circularity or pyknotio nucleolus came forth. Conclusions: Alarelin may have short-term therapeutic effect for endometr ial cancer, especially for endometrioid cancer It may also inhibit the synthetic metabolism and mitosis of cancer cell.