摘要
目的：探讨谷胺甘肽Ｓ－转移酶、脱氧核糖核酸拓扑Ⅱ的表达与晚期宫颈癌化疗的关系。方法：应用免疫组化Ｓ－Ｐ法检测２８份晚期宫颈癌组织化疗前后ＧＳＴ－π和ＤＮＡＴｏｐｏⅡ的表达。结果：化疗前２８份宫颈癌组织中ＧＳＴ－π过表达１７份（６０．７１％)，ＤＮＡ ＴｏｐｏⅡ低表达１６份（５７．１４％）；化疗后ＧＳＴ－π过表达２４份（８５．７１％），ＤＮＡ ＴｏｐｏⅡ低表达１８例（６４．２８％）。化疗前后ＧＳＴ－π的表达差异有显著性（Ｐ＜0．05），ＤＮＡ ＴｏｐｏⅡ的表达差异无显著性（Ｐ＞０．０５），二者表达有相关性（Ｐ＝０．０１９）。ＧＳＴ－π过表达和ＤＮＡＴｏｐｏⅡ低表达与患者的化疗反应呈负相关（Ｐ＝０．０１４，Ｐ＝０．０３８）。结论：ＧＳＴ－π过表达与宫颈癌内在性耐药和获得性耐药有关，ＤＮＡ TｏｐｏⅡ低表达只与宫颈癌内在性耐药有关。ＧＳＴ－π和ＤＮＡ TｏｐｏⅡ的表达均能很好地预测晚期宫颈癌患者的化疗耐药与反应。
Objective:To study the relationships between expressions of GS T-π and DNATopoⅡ and chemotherapy of pat ient with advanced cervical cancerM ethods:The expre ssions of GST-π and DNA TopoⅡ in 28 adva nced cervical cancer before and after ch emotherapy were studied by means of S-P immunohistochemistry.Results:O verexpressions of GST-π were seen in 60. 71%(17/28) and 85.71%(24/28) of advanced cervical cancer before and after chemot herapy,respectively.There was a signific ant difference between them(P<0.05 ).Lowexpressions of DNA Topo Ⅱ wer e seen in 57.14%(16/28) and 64.28%(18/28 ) of advanced cervical cancer before and after chemotherapy,respectively.There w as no significant difference between them (P>0.05).A correlation of expressions between GST-π and DNA Top o Ⅱ was observed (P=0019)The re were negative relationships between o verexpressio n of GST-π,lowexpression of DNATopo Ⅱ an d chemoresponsiveness of cervical cancer (P=0.014,P=0.038).Conclusions: The overexpression of GST-π and lowexpression of DNA Topo Ⅱ may contribute to th e intrinsic drugresistance in cervical cancer. Moreover, cervical cancer may also acquire drugresistance by chemotherapy through the enhancement of GST-π expressi on. Levels of GST-π and DNATopo Ⅱ express ion can both predict chemotherapeutic an d response of cervical cancer well.
出处
《现代妇产科进展》
CSCD
2000年第4期250-252,共3页
Progress in Obstetrics and Gynecology
