肺癌患者血清MMP-9和TIMP-1检测的临床意义

The clinical significance of the measurement of serum MMP-9 and TIMP-1 in patients with lung cancer

目的研究基质金属蛋白酶-9(MMP-9)和组织金属蛋白酶抑制剂-1(TIMP-1)在肺癌患者和健康人血清中的含量,及其与分期、肿瘤分化程度、肿瘤大小、淋巴结转移状态、远处转移等病理特征的相关性。方法通过酶联免疫吸附测定(ELISA)分别检测肺癌患者和健康对照者的血清样本中的MMP-9、TIMP-1的浓度,进行统计学分析。结果 MMP-9和TIMP-1在肺癌患者血清中的含量明显高于健康人群,差异有统计学意义(P<0.01);并与分期、肿瘤大小、淋巴结转移、远处转移呈正相关(P<0.05),与分化程度呈负相关(P<0.05);而与年龄、性别、吸烟史、肿瘤的病理类型无关(P>0.05)。结论 MMP-9和TIMP-1参与肺癌的发生、发展、侵袭转移过程,可作为临床监测病情发展的指标。

Objective To investigate the serum expression of matrix metalloproteinase-9（MMP-9） and tissue inhibitors of matrix metalloproteinase-1（TIMP-1） in lung cancer patients and healthy people,and its relationship with clinical pathological features,including staging,the degree of tumor differentiation,the size of tumor,the lymph node metastasis status and distant metastasis,etc.Methods The serum expression of MMP-9 and TIMP-1 in lung caner patients and healthy people were tested by ELISA and analyzed statistically.Results The serum expression of MMP-9 and TIMP-1 in lung caner patients were significantly higher than that of healthy people（P0.01）,and the levels of MMP-9 and TIMP-1 was positively related with staging,tumor size,lymph node metastasis and distant metastasis（P0.05）,and they were negatively related with the degree of differentiation（P0.05）.The levels of MMP 9 and TIMP 1 were not related with age,gender,smoking history and pathological type（P0.05）.Conclusion MMP-9 and TIMP-1 may take part in the progress of lung cancer,and could be used as clinical indicators to monitor the development of disease.