高效液相色谱法测定马兰中大黄素的含量

Determination of emodin in Kalimeris indica(L. ) Sch-Bip by HPLC

目的建立测定中草药马兰中大黄素含量的高效液相色谱方法。方法甲醇回流提取药材,高效液相色谱法测定,采用的色谱柱为Kromasil-C18柱(250 mm×4.6 mm,5μm);流动相为甲醇-0.1%磷酸水溶液(80∶20);检测波长为254 nm;柱温为30℃;流速为1 mL.min-1。结果大黄素溶液在1.4～14 mg.L-1浓度范围内与其峰面积呈良好线性关系(r=0.999 3),平均加样回收率为99.61%,其RSD值为1.08%(n=6)。结论该方法准确、可靠、灵敏、简便,适合中草药马兰中大黄素含量的测定。

Objective To establish an HPLC method for determination of emodin in Kalimeris indica（L.）Sch-Bip.Methods The dry medicinal herb was extracted by refluxing in methanol and determined by HPLC.A Kromasil-C18 column was used with the mobile phase composed of methanol-phosphoric acid solution（0.1%）（80∶ 20） at the flow rate of 1 mL·min-1.The detection wavelength was 254 nm and the column temperature was 30 ℃.Results Emodin showed a good linear relationship to the peak aera at a concentration range of 1.4~14 mg·L-1（r=0.999 3）.The average recovery was 99.16% with RSD of 1.08%（n=6）.Conclusion The method is accurate,reliable,sensitive,simple and suitable for the determination of emodin in Kalimeris indica（L.）Sch-Bip.