植物磺肽素在杂交鹅掌楸体胚发生中的作用

Effects of Phytosulfokine on the Somatic Embryogenesis of Liriodendron hybrids(L. chinense×L. tulipifera)

植物磺肽素(PSK),是近年来发现的一种新型植物肽类生长调节物质,具有促进单细胞的分裂增殖等生理功能。以杂交鹅掌楸未成熟胚来源的体胚发生体系为材料,探索PSK-α在植物体胚发生中的作用。研究发现PSK-α能够促进细胞分裂和增殖,有利于胚性细胞的形成。在杂交鹅掌楸体胚发生初期添加0.1mg·L-1的PSK-α可提高未成熟胚的脱分化率并能有效改善培养细胞的状态;0.5mg·L-1的PSK-α能够促进胚性愈伤组织的形成和增殖;体胚形成后期添加0.8mg·L-1的PSK-α利于其正常发育以及再生植株的培养。

Phytosulfokine （PSK）,a new type of plant growth regulator, has multiple biological activities besides promoting single cell proliferation. In this paper, we imvestigated the role of PSK-α during somatic embryogenesis of Liriodendron hybrids（L. chinense×L. tulipifera） using immature zygotic embryos as explants. The result showed that PSK-α was able to improve cell division and proliferation and induce embryogenic cells formation. At the early stage of somatic embryogenesis, 0.1 mg·L-1 PSK-α increased the ratio of callus formation from explants and improved cell state; 0.5 mg·L-1 PSK-α induced embryogenic callus formation; and 0.8 mg·L-1 PSK-α was in favor of somatic embryo development and growth of regeneration plantlets.