纤维素酶预处理对葛渣异黄酮提取的影响

Effect of Cellulase Pretreatment on Isoflavones Extraction from Pueraria Root Residue

【目的】对纤维素酶预处理提取葛渣异黄酮的条件进行优化。【方法】在单因素试验基础上,采用响应面分析法研究纤维素酶量、酶解温度、pH值和酶解时间对异黄酮得率的影响,优化提取条件。【结果】建立了纤维素酶量、酶解温度、pH值、酶解时间与异黄酮得率之间的回归模型,得到纤维素酶预处理的最佳条件:纤维素酶用量11mg(以5g葛渣计),酶解温度51℃,pH 5.0,酶解时间2.3h,异黄酮得率可达12.34mg/g,比传统醇提法得率提高57%。【结论】纤维素酶预处理提取葛渣异黄酮效果较好,异黄酮得率较高。

【OBJECTIVE】The objective of this study was to optimize the extraction conditions with cellulase pretreatment of isoflavones from pueraria root residue. 【METHOD】Single-factor test and response surface methodology （RSM） were used to analysis the effects of the enzyme dosage, enzymolysis temperature, pH value and enzymolysis time on the extraction yield of isoflavones.【RESULTS】The optimal enzymolysis conditions were got through the regression model, which are cellulase dosage 11mg per 5g pueraria root residue, enzymolysis temperature 51℃, pH5.0, enzymolysis time 2.3h. Under these conditions, the extraction yield of isoflavones can reach at 12.34mg/g, which is increased 57% than that of traditional method. 【CONCLUSION】The result shows that the optimal conditions with cellulase pretreatment can effectively improve the extraction amount of isoflavones from pueraria root residue.