旋毛形线虫预感染对夏氏疟原虫感染BALB/c小鼠Th细胞变化的影响

Effect of Trichinella spiralis Pre-Infection on Alterations in BALB/c Mice Th Cell Infected with Plasmodium chabaudi chabaudi AS

为研究预感染旋毛形线虫后BALB/c小鼠抵御夏氏疟原虫攻击感染的能力,实验组(TPC)分别在小鼠感染旋毛形线虫1 w(TPC1)和3 w(TPC3)后感染夏氏疟原虫,对照组(PC)单独感染夏氏疟原虫,观察小鼠原虫血症及体重的变化,并采用Real-Time RCR扩增法分析转录因子T-bet和Gata-3表达水平的消长。与PC组相比,TPC1组原虫血症峰值降低,T-bet表达水平升高,Gata-3表达水平降低;TPC3组原虫血症出现时间推迟,峰值升高,T-bet表达水平降低,Gata-3表达水平升高;TPC组体重均下降,TPC组与PC组均于感染后25 d左右自愈。旋毛虫预感染使小鼠对疟原虫的抗感染能力增强,但混合感染并不影响宿主最终清除疟原虫的能力。

The resistant ability of BALB/c mice pre-infected with Trichinella spiralis against P. chabaudi chabaudi AS infection was studied. Experimental groups （TPC） were infected with T. spiralis, then infected with P. c chabaudi AS lw （TPC1） and 3w （TPC3） after T. spiralis infection respectively, the control group （PC） was only infected with P. c chabaudi AS, parasitemia was detected and weight was measured, moreover, the growth and decline of the expression level of T-bet and Gata-3 transcription factors was detected using Real-Time PCR. As compared with PC group, TPC1 group declined its peak of parasitemia, the expression level of T-bet gene was higher while Gata-3 gene was lower. However, the peak-reaching time of parasitemia was delayed in TPC3 group, and a higher peak-para- sitemia occurred, the expression level of T-bet gene rose, while the expression level of Gata-3 gene rose. Both TPC groups lost weight, and TPC and PC groups showed autotherapy about day 25 after infection. These results suggested that the anti-infection ability of BALB/c mice increased after pre-infection with T. spiralis, however, mixed infection did not affect the ability of the host finally to remove plasmodia.