玉米抗矮花叶病基因SSR分子标记定位研究

Location of maize dwarf mosaic virus-resistant genes with SSR makers

通过矮花叶病抗性鉴定筛选出玉米高抗自交系黄野四-3和高感自交系8112。遗传分析显示矮花叶病抗性表现为显性遗传。利用SSR分子标记,结合群体分离分析方法(BSA),对抗矮花叶病基因进行定位,筛选获得了与玉米抗矮花叶病基因位点连锁的SSR标记,并将该基因定位于第3连锁群,与两侧的Bnlg1035和Umc2266分子标记遗传距离分别为8.02 cM和3.04 cM。这一研究结果为快速筛选抗矮花叶病玉米新种质,培育抗性新品种提供了理论依据,为抗矮花叶病基因的分子标记辅助育种和抗病基因克隆奠定了基础。

In this study,we screened out highly resistant Huangyesi-3 and Susceptible 8112 maize inbred lines by identification of resistance to maize dwarf mosaic virus（MDMV）.Genetic analysis showed that the resistance to MDMV was autosomal dominant.The resistant genes were located by BSA（bulked segregate analysis） method using SSR molecular markers.SSR molecular markers that linked tightly to the resistant gene were screened and located accurately on chromosome 3.The linkage distance between Bnlg1035 and Umc2266 are 8.02 cM and 3.04 cM,respectively.These results provide a theoretical basis for screening the resistant germplasm quickly and cultivating new varieties,and lay a foundation for molecular marker-assisted breeding and cloning of resistance gene to maize dwarf mosaic virus.