HPLC法测定木蝴蝶中木蝴蝶苷A和木蝴蝶苷B的质量分数

HPLC Determination of Oroxin A and Oroxin B in Oroxylum indicum(L.) Vent

建立了利用高效液相色谱法(HPLC)同时测定木蝴蝶中木蝴蝶苷A和木蝴蝶苷B的定量分析法:采用COSMOSIL-C18-MS-Ⅱ(4.6 mm×250 mm,5μm)色谱柱,以V(甲醇)∶V(ρ(磷酸)=0.2%)=4∶6为流动相进行洗脱,流速1.0 mL/min,检测波长278nm,柱温25.0℃.结果表明:木蝴蝶苷A的线性范围为0.050～2.0μg(r2=0.999 9),平均回收率为101.14%,相对标准偏差(RSD)为1.07%;木蝴蝶苷B的线性范围为0.050～2.0μg(r2=0.999 9),平均回收率为100.41%,RSD为2.26%.

An HPLC method was used for the determination of Oroxin A and Oroxin B in Oroxylum indicum（L.） Vent.A COSMOSIL-C18-MS-Ⅱ（4.6 mm×250 mm,5 μm） column was adopted.The mobile phase consisted of V（methanol）∶V（φ（phosphoric acid water）=0.2%）=4∶6,at a flow rate of 1.0 mL/min.The wavelength of VWD detector was set at 278 nm and the column temperature was 25.0 ℃.The linear range of Oroxin A was 0.05—2.0 μg（r2=0.999 9）,and the mean recovery was 101.14%（RSD=1.07%,n=6）;the linear range of Oroxin B was 0.050—2.0 μg（r2=0.999 9）,and the mean recovery was 100.41%（RSD=2.26%,n=6）.