摘要
目的探讨不同的保存时间、温度对类风湿关节炎(RA)患者外周血与关节积液IL-18水平的影响。方法将12例RA患者的血清与关节积液按照不同保存时间与温度条件分为A组(血清,室温0h)、B组(全血,室温36h)、C组(全血,室温72h)、D组(全血,4℃72h)、E组(血清,室温72h)、F组(关节液,室温0h)、G组(关节液,室温72h),用ELISA检测各组IL-18浓度变化。结果 B、D、E组IL-18浓度明显高于A组(均P<0.05);F组IL-18浓度与A组比较差异无统计学意义(P>0.05)。非参数两关联样本检验显示C组IL-18浓度明显高于A、B、D组;G组IL-18浓度明显高于F组(P<0.05)。结论外周血及关节积液IL-18水平变化趋势一致,IL-18检测受时间和温度影响,且温度影响更显著。
Objective To study the influence of different preservation time and temperature on the IL-18 level of peripheral blood and joint effusion in the patients with rheumatoid arthritis（RA）. Methods Serum and joint effu- sion in 12 cases of RA were divided into the group A（serum at room temperature for 0 h）,g（whole blood at room temperature for 36 h）,C（whole blood at room temperature for 72 h）,D（whole blood at 4 ℃ for 72 h）,E（serum at room temperature for 72 h）, F（joint fluid at room temperature for 0 h） ,G（joint fluid at room temperature for 72 h） according to the different storage time and temperature conditions. The changes of IL-18 concentration in various groups were detected by ELISA. Results The IL-18 concentration in the group B,D and E was significantly higher than that in the group A（all P〈0.05）. The IL-18 concentration had no statistical difference between the group A and F（P〉0.05）. Non-parametric two-related sample test showed that the IL-18 concentration in the group C was signifi- cantly higher than that in the group A,B and D;which in the group G was significantly higher than the group F（P〈0.05）. Conclusion The change of IL-18 level in peripheral blood and joint effusion demonstrates the consistent trend,the IL-18 detection is affected by the time and temperature, especially temperature influence is more signifi- cant.
出处
《检验医学与临床》
CAS
2013年第6期652-653,共2页
Laboratory Medicine and Clinic
