流式荧光杂交法检测人乳头瘤病毒的临床应用

Clinical application of flowcytometry fluorescence hybridization method in human papillomavirus genotype detection

目的探讨流式荧光杂交法检测人乳头瘤病毒(HPV)的临床应用价值。方法采集苏州地区1 406例妇科就诊患者宫颈脱落细胞,用流式荧光杂交技术检测HPV基因型,并对HPV感染率、基因亚型和年龄分布等进行分析。结果检出HPV感染576例(40.97%),其中单一亚型感染392例(27.88%),多重亚型感染184例(13.09%);采用流式荧光杂交法可同时检测26种HPV基因亚型,最常见的亚型为HPV-6、11、16、52、58等;单纯HPV低危亚型感染117例,占阳性病例的20.31%,高危亚型感染365例(63.37%);50～59岁年龄组HPV感染率最高(52.24%),30～39岁年龄组感染率最低(34.02%);并且,随年龄增加低危亚型感染率逐步降低,高危亚型感染率却呈明显上升趋势。结论本地区妇科患者中HPV感染率较高,流式荧光杂交法是检测HPV感染的有效方法,开展HPV分型检测对宫颈病变的防治意义重大。

Objective To discuss the clinical application of flowcytometry fluorescence hybridization method in human papillomavirus genotype detection. Methods The cervical cast off cells of 1 406 patients in Suzhou area were collected,and the HPV genotypes of the samples were analyzed with flowcytometry fluorescence hybridization meth- od. The HPV infection rate, infectious status of various genotypes and age distribution of HPV were analyzed as well. Results 576 cases were positive（40.97%） ,in which single genotype infection rate was 27.88%（392/576） ,and multiple genotypes infection rate was 13.09% （184/576）. The 26 genotypes of HPV could be tested simultaneously with flowcytometry fluorescence hybridization method, and among them the common subtypes were HPV-6,11,16, 52,58, etc. Among all the HPV-infected patients, 117 cases （20.31%） were just low risk genotype infection, while high risk HPV infection was in 365 cases（63.37%）. The highest HPV infection rate was found at the age group of 50-59 years（52.24%） and the lowest HPV prevalence was found at the age group of 30-39 years（34. 02%）. Along with the increasing age,the infection rate of high risk HPV was increased gradually and the low risk HPV was de- creased. Conclusion The positive rate of HPV infection in local area is rather high. flowcytometry fluorescence hy- bridization method is the most effective method to detect the HPV infection,and it can be of great value to the pre- vention and treatment of cervical diseases.