摘要
[ Objective] The aim was to select protective agent of HA antigen of influenza virus. [ Method] H3N2 and H1 N1 subtype influenza virus were added into A - F groups to conduct accelerated test at 37 ℃and measure HA titer through hemagglutination test. t Result] Hemagglutination titers of H3 N2 and H, N1 subtype influenza virus were 20 and 32 in group A （ with PBS buffer solution） for 28 d; heat stability of HA antigen proved the best in group F （with BSA, fucose, proclin 300, triton 100） ; hemagglutination titers of HA antigen of H3N2 and H1 N1 subtype virus were 48 and 96 for 28 d. [ Conclusion] Components in group F were best in protection on HA antigen, which can be a candidate of protective agent.
[ Objective] The aim was to select protective agent of HA antigen of influenza virus. [ Method] H3N2 and H1 N1 subtype influenza virus were added into A - F groups to conduct accelerated test at 37 ℃and measure HA titer through hemagglutination test. t Result] Hemagglutination titers of H3 N2 and H, N1 subtype influenza virus were 20 and 32 in group A （ with PBS buffer solution） for 28 d; heat stability of HA antigen proved the best in group F （with BSA, fucose, proclin 300, triton 100） ; hemagglutination titers of HA antigen of H3N2 and H1 N1 subtype virus were 48 and 96 for 28 d. [ Conclusion] Components in group F were best in protection on HA antigen, which can be a candidate of protective agent.
基金
funded by the National863Plan-Platform Establishment of Production Technology of Pandemic Vaccine by Vero Cell Carrier(2006AA02Z409)
Yunnan Natural Science Foundation-Vero Cell Adapted Strains of Influenza A virus(2004C070M)
Yunnan Domestic S&T Cooperation Project-Development of Influenza Virus Vaccine of Vero Cell(2006YX29)
