SLE患者外周血单个核细胞中信号转导子及转录激活因子4mRNA的表达及其与血清IFN-γ水平之间的相关性

Correlation of mRNA level of STAT4 in peripheral blood mononuclear cells with IFN-γ level in serum in patients with systemic lupus erythematosus

目的探讨系统性红斑狼疮(SLE)外周血单个核细胞(PBMC)中信号转导子及转录激活因子4(STAT4)mRNA表达及其与血清干扰素(IFN-γ)水平之间的相关性。方法用TaqMan实时荧光定量聚合酶链反应法检测60例SLE患者及30例正常对照者PBMC中STAT4mRNA表达水平,用酶联免疫吸附法检测SLE患者血清中IFN-γ的水平,并分析两者之间的相关性。结果 PBMC中STAT4mRNA表达水平在SLE患者组中要显著高于正常对照组(P<0.05),并且在SLE患者病情活动组中显著高于病情非活动组(P<0.05)。SLE患者血清中IFN-γ水平检测结果为(12.71±5.68)ng/ml,并与患者PBMC中STAT4mRNA表达呈正相关(r=0.769,P<0.05)。结论 SLE患者PBMC中STAT4mRNA的表达水平高于正常,并与病情活动性及患者血清中IFN-γ水平相关。

Objective To explore the correlation of mRNA level of STAT4 in peripheral blood mononuclear cells （PBMC） with IFN-γ level in serum in patients with systemic lupus erytherrratosus （SLE）. Methods Peripheral blood samples from patients with SLE （n=60, the study group） and healthy individuals （n=30, the control group） were used to detect STAT4 mRNA level by TaqMan real-time RT-PCR and to evaluate IFN-γ level in serum by ELISA methods. Results The STAT4 mRNA level was significantly higher in the study group than the control group, and was also significantly higher in active SLE patients than in non-active SLE patients （P〈0.05）. The IFN-γ level in se-rum was （12.71±5.68）, and was positively associated with STAT4 mRNA level （r=0.769, P〈0.05）. Conclusion A sig-nificant correlation is found between mRNA level of STAT4 in PBMC and the disease activity or IFN-y level in se-rum in patients with SLE.