不同剂量X射线对雄性大鼠精子顶体酶活性的影响

Effecet of Different Doses of X-ray on Acrosomal Enzyme Activities of Spermatozoa of Male Rats

目的:检测不同剂量X-射线对雄性大鼠精子顶体酶的影响,以探讨体外X-射线对大鼠精子顶体酶活性的影响。方法:将10周龄成熟雄性大鼠40只,随机分为4组:2Gy、4Gy、6Gy照射组和0Gy对照组;每组10只。按不同剂量照射,1h后处死大鼠,以改良的Kennedy法分别测定不同剂量X射线作用下的精子顶体酶活性。结果:2Gy、4Gy、6Gy照射组与0Gy对照组相比顶体酶活性显著降低(P<0.05),照射组酶活性较0Gy对照组有显著降低;4Gy、6Gy照射组与2Gy照射组相比较顶体酶活性显著降低(P<0.05);而4Gy照射组与6Gy照射组相比较则无统计学意义。结论:X-射线对大鼠精子顶体酶有明显的抑制作用。

Objective To detect the effect of different doses of X-ray on acrosomal enzyme activities of spermatozoa of male rats in order to explore the effect of X-ray exposure on acrosomal enzyme activities of spermatozoa in vitro. Methods d0 male SD-rats of 10 weeks were randomly divided into 4 guoups： X-ray irradianted groups：including 2Gy, 4Gy, 6Gy and 0Gy control group. All rats were irradiated and killed after lh. The acrosomal enzyme activities were measured by the improved Kennedy method. Results Com- pared with 0Gy control group, the sperm acresomal enzyme activity in 2Gy, 4Gy and 6Gy groups treated with high doses of X-ray de- creased significantly （P 〈0.05）. Compared with 2Gy irradiated group, the sperm acrosomal enzyme activity in 4Gy and 6Gy groups treated with high doses of X-ray decreased significantly （P 〈 0.05 ） ; the sperm acrosomal enzyme activity in 4Gy and 6Gy irradiated groups showed nostatistical significance. Conclusion X-ray has obvious dose-dependent inhibitive effects on the acrosomal enzyme activity of spermatozoa of male rats.