散尾葵拟盘多毛孢叶斑病菌的鉴定

Identification of Pathogen Causing Leaf Spot of Chrysaliclocarpus lutescens

采集田间自然发病典型的散尾葵叶部,按照柯赫氏法则获得致病菌株,通过病原菌形态观察以及核糖体DNA-ITS序列分析,结果表明,散尾葵叶斑病的病原菌为小孢拟盘多毛孢[Pestalotiopsis microspora(Speg.)Satista&Peresapud Batista]。

Chrysaliclocarpus lutescens leaf spot is a Chrysaliclocarpus lutescens＇s disease which pathogenic fungi infected the leaf margin or tip. In the early days, the spots are brown dots, gradually expanding into oval or irregularly-shaped, dark or grey in the middle of lesion, dark lines around the edge. Late dispersed oval dots. This disease can cause the whole plant leaf wilted and crispated. This disease seriously affects the normal growth of Chrysaliclocarpus lutescens. The pathogenic isolate was obtained according to the Koch＇s postulate. Through observing the pathogenicity, the hyphal form, the sequence of ribosomal DNA-ITS, and the morphological characteristics such as size, colour and so on. The pathogen was identified as Pestalotiopsis microspora （Speg.） Satista ＆ Peresapud Batista.