四川、重庆不同紫苏遗传多样性的SRAP研究

On Genetic Diversity of Perilla Frutescens(Linn). Britt in Sichuan and Chongqing by SRAP Marker

目的:研究四川重庆不同紫苏材料的遗传多样性,为种质资源的分类和选育打下理论基础.方法:采用SRAP分子标记方法对来自四川、重庆的10份紫苏材料进行遗传多样性分析,利用popgene1.32及NTSYS-pc2.1软件处理数据,UPGMA方法聚类,构建亲缘关系树状图.结果:26对SRAP引物组合扩增出167条带,多态比率(PPB)为51.57%.10份材料的遗传距离变化范围为0.098 8～0.916 3,平均为0.490 8.用籽材料中,四川和重庆地区的平均多态位点百分率为27.25%,在两个地区间,SRAP标记的平均H值分别为0.149 6和0.232 8.平均I值为0.221 1和0.350 8.结论:SRAP分子标记适合用于紫苏材料的遗传多样性分析,能够清晰地揭示不同材料间的亲缘关系,为下一步紫苏的良种选育提供科学依据.

Objective of the study：The genetic diversity of Perilla frutescen（Linn）.Britt in Sichuan and Chongqing has been researched in this paper to make Chongqing estimated by means of sequence-related amplified polymorphism（SRAP）.The date has been analyzed by the theoretical basis for promising germplasm of identification and further breeding.Methods of the study： Ten materials in Sichuan and Popgene 1.32 and NTSYS-pc2.1 software.The system diagram of genetic relationship has been built by UPGMA.Results of the study：26 pairs of SRAP primers combination amplified 167 polymorphic bands,and the average percentage of polymorphic bands reaches to 51.57%.The Nei＇s genetic identity of 10 samples ranges in 0.098 8-0.916 3.The average is 0.490 8.The average of percentage of polymorphic bands of materials used breeds in Sichuan and Chongqing is 27.25%.Among the two areas： The average of Nei＇s gene diversity（H） is 0.149 6 and 0.232 8 by SRAP marker.The average of Shannon＇s information index（I） is 0.221 1 and 0.350 8 by SRAPs.Conclusion of the study： SRAP markers can be appropriately applied to genetic analysis in Perilla frutescens（Linn）.Britt.It can possess several genetic relationships syllabified to provide the scientific basis.