应用RT-PCR和IC-RT-PCR方法检测南瓜花叶病毒

Development of a novel RT-PCR and IC-RT-PCR method for detecting Squash mosaic virus

南瓜花叶病毒(Squash mosaic virus,SqMV)是葫芦科作物上一种重要的种传病毒。本研究根据其外壳蛋白(CP)基因设计一对特异引物,建立了SqMV的RT-PCR和IC-RT-PCR检测方法。特异性检测结果表明,所建立的方法可将SqMV与同一个病毒属的其他病毒及健康寄主植物准确区分开来,具有良好的特异性。在此基础上,建立了SqMV的IC-RT-PCR检测方法,其灵敏度则比DAS-ELISA方法提高10倍以上。所建立的方法具有良好的特异性和灵敏度,适用于进出境植物繁殖材料的快速检测。

A novel reverse transcription - polymerase chain reaction（RT-PCR）method was developed to detect Squash mosaic virus （SqMV）. The assay specificity was evaluated on other comoviruses and heathy host plants. The IC - RT - PCR assay was also developed and it was up to 10 times more sensitive than DAS - ELISA in detecting SqMV. The method in this study is suitable for fast, specific and sensitive detection of SqMV.