基于ITS1序列的鸡艾美耳球虫种的套式PCR鉴别方法

Establishment of a nested-PCR assay for discriminating chicken Eimeria species based on ITS1 sequences

为快速诊断鸡艾美耳球虫感染,基于ITS1序列建立了鸡艾美耳球虫套式PCR鉴别检测方法。该方法实现了对鸡柔嫩艾美耳球虫、堆型艾美耳球虫、早熟艾美耳球虫、和缓艾美耳球虫、毒害艾美耳球虫和布氏艾美耳球虫间的快速鉴别。测序结果和序列比对分析显示ITS1序列具有良好的种特异性,可用于艾美耳球虫属下各虫种的鉴别诊断和分类学研究,基于ITS1序列建立的套式PCR方法为艾美耳球虫鉴别诊断和分子流行病学研究提供了新的工具。

To diagnose coccidiosis of chicken in a fast manner, a nested-PCR assay on the basis of the ribosomal internal transcribed spacer 1 （ITS1） sequence was established to discriminate six chicken Eirneria species：E, tenella, E. mitis, E. necatri：c, E. acervulina, E. praecox, and E. brunetti. Sequencing of PCR amplicons combined with the subsequent sequence analysis indicated ITS1 was able to serve as a species- specific diagnostic marker for the taxonomic research of Eimeria. This study provided a novel tool for the rapid discrimination of Eimeria species, which may be helpful in Eimeria epidemiological investigation.