从微血管内皮细胞转运功能探讨针刺对缺血性中风的影响机制

Study of the effects of acupuncture from the transporting function of microcascular endothelium cell on the rats with focal cerebral ischemia

目的:通过研究针刺对脑缺血大鼠侧脑室微量注射葡萄糖转运蛋白(Glut-1)、P-糖蛋白(P-gp)阻滞剂后微血管内皮细胞转运功能的影响,探讨针刺是否通过影响微血管内皮细胞的Glut-1和P-gp的表达,减轻缺血后神经细胞的损伤,从而保护缺血脑组织,为针刺治疗脑缺血性疾病提供实验及理论依据。方法:采用大脑中动脉闭塞法制备局灶性脑缺血模型,侧脑室微量注射5-硫D-葡萄糖、环孢菌素A后,观察电针内关穴(每天1次)7d后脑组织葡萄糖、乳酸、丙酮酸、Ca2+和谷氨酸的含量;免疫组织化学方法检测Glut-1、P-gp的表达。结果:针刺可提高脑组织葡萄糖含量,降低乳酸、谷氨酸、Ca2+含量,促进Glut-1、P-gp表达。侧脑室注射Glut-1、P-gp阻滞剂后可抑制内皮细胞转运功能,减低针刺的上述效应。结论:针刺具有改善脑缺血后微血管内皮细胞转运功能,减轻缺血后神经细胞损伤的作用。针刺治疗脑缺血机制可能与脑微血管内皮细胞转运功能有关。

Objective： To study acupuncture stimulation on the transporting function of microvascular endothelial cells after the intracerebroventricular microinjection of Glut-1,P-gp blockers in rats with focal crebral ischemia.Whether acupuncture could reduce the ischemic nerve cell damage and protect the ischemic brain tissue by affecting Glut-1 and P-gp expression in the microvascular endothelial cells was explored in the present study.Methods： Cerebral ischemia animal models were induced by middle cerebral artery occlusion with thread embolus in Wistar rats.After ivc 5-thio-D-glucose and cyclosporine A,electroacupuncture was applied to bilateral ‘Neiguan’（PC 6） for seven days.The content of Glucose,Pyruvic Acid,Lactic Acid,Ca2＋ and Glutamate in brain tissue was analyzed with specialized measuring kits.The expression of Glut-1 and P-gp in hippocampal CA1 area was observed with the method of immuno-histochemical.Results： The content of Lactic Acid,Ca2＋ and Glutamate in brain tissue were decreased after acupuncturing at Neiguan.The content of Pyruvic Acid and Glucose was increased in Neiguan groups.Acupucture can improve the expression of Glut-1 and P-gp in hippocampal CA1 region of MCAO rats.Conelusion： The results demonstrate that EA improves the transporting function of microvascular endothelial cells and reduces the injured neurons in rats with focal cerebral is chemia.