狭小空间晕动病模型的建立及其防治中药的研究

Establishment of motion sickness model in narrow space and study of traditional Chinese medicine on prevention and treatment

目的:建立狭小空间小鼠晕动病模型,筛选有效防治晕动病的中药。方法:以振荡器为模型,采用L9(34)正交表,以频率、时间、温度、拥挤程度为因素,小鼠爬杆时间及粪便、尿液、立毛、颤抖等生理指标为评价标准,建立晕动病模型,结合小鼠血浆促肾上腺皮质激素(ACTH)和β-内啡肽(β-EP)的变化验证其效果,以此模型评价10种中药药效。结果:温度30℃,以280r/min的频率、452.16cm2/只的拥挤程度持续40min可达到最佳的模型效果。生姜、丁香、菊花等实验组小鼠的爬杆时间和生理指标均低于模型对照组和阳性对照组,且差异显著(P<0.05)。结论:振荡器可作为狭小空间晕动病的理想模型;生姜、丁香、菊花具有较好的防治晕动病效果。

Objective： To build the mice motion sickness model in narrow space,and choose the Chinese medition of effective prevention and treatment.Methods： We choosed the Kunming mice and L9（34） orthogonal table,and frequency,time,temperature,level of crowding as factors,put pole climbing time and physiological index of stool,urine,piloerection and trembling as the evaluation index.And then we detected the plasma levels of ATCH and β-EP.We built motion sickness model,to evaluate the efficacy of ten kinds of Chinese medicines.Results： The best process was as follows： oscillation frequency of 280r/min,oscillation time of 40min,experimental temperature of 30°C,crowding level of 452.16 cm3 every.The index of ginger,cloves,chrysanthemums were significantly lower than the model group and drug group（P0.05）.Conclusion： Oscillator is an effective narrow space motion sickness model;ginger,clove and chrysanthemum have the better anti-halo effect.