PARP抑制剂对非小细胞肺癌A549及H460细胞增殖及凋亡的影响

Effect of PARP inhibitor PJ34 on cell proliferation and apoptosis of non-small cell lung cancer cell line A549,NCI-H460

目的:探讨PARP抑制剂PJ34体外对非小细胞肺癌的增殖及凋亡影响。方法:采用MTT法检测PARP抑制剂PJ34对非小细胞肺癌A549、NCI-H460细胞的增殖抑制作用;Hoechst33342荧光染色法观察PJ34诱导的细胞凋亡;流式细胞术检测PJ34作用后细胞周期变化及凋亡情况。结果:在1.25-20μg/ml浓度范围内,PJ34抑制A549、NCI-H460细胞增殖,且具有浓度和时间依赖性。荧光显微镜下观察到PJ34处理后细胞出现凋亡的形态学变化如核固缩、体积缩小等。细胞周期分析PJ34能将细胞阻滞于细胞周期中G0/G1期。Annxin V-FITC/PI双染流式细胞分析发现PJ34作用后细胞凋亡比例明显增加(P<0.01)。结论:PARP抑制剂PJ34能够抑制非小细胞肺癌A549、NCI-H460细胞增殖,引起细胞周期阻滞、诱导细胞凋亡。

Objective：To investigate the effects of PARP inhibitor PJ34 in vitro on the proliferation and apoptosis of non - small cell lung cancer cell hnes A549, NCI - H460. Methods： The inhibition rates of A549 and H460 ceils exposed to PJ34 were evaluated by MTT method. The morphological changes of apoptosis induced by PJ34 were observed by Hoechst33342 fluorescence staining under the fluorescence microscope. Cell cycle and apoptosis changes of the cell lines treated by PJ34 was detected by flow cytometry. Results ：The MTr assay showed PJ34 had significantly antiproliferative effects with a dose and time - dependent way at the concentration ranged from 1.25 to 20μg/ml. Typical apoptotic morphologic changes were observed by fluorescence microscope, such as nuclear condensation and chro- matin condensation. Flow cytometry assay showed that cell cycle was arrested at the G0/GI phase, Annexin V - FIT（I/ PI double staining flow cytometry analysis showed that the apoptosis was significantly increased after PJ34 treatment （ P 〈 0.05 ）. Conclusion： PARP inhibitor PJ34 can inhibit non - small cell lung cancer A549, H460 cell proliferation, and cause cycle arrest and induce apoptosis.