联用大黄素、黄芩苷对牙周炎大鼠炎症因子影响的研究

Effects of emodin in combination with baicalin on inflammatory factor in rats with periodontitis

目的观察大黄素、黄芩苷联用对牙周炎大鼠牙龈组织炎症因子的影响。方法采用钢丝结扎配合高糖饮食喂养法建立大鼠牙周炎模型,随机分为空白对照组、模型组、替硝唑治疗组、黄芩苷治疗组、大黄素治疗组、黄芩苷与大黄素联用组。分别于实验第4、8周处死,取牙龈组织匀浆,ELISA法检测TNF-α、IL-6含量。结果大鼠建模后牙周炎症状明显,组织TNF-α、IL-6含量均不同程度升高,其中模型组升高最显著,联用组在4、8周时间点牙龈组织TNF-α和IL-6含量均较大黄素组明显降低(P<0.05),而与黄芩苷组比较,在早期(4周时间点)TNF-α和IL-6含量显著降低(P<0.05),但8周时2组无显著性差异。结论大黄素和黄芩苷联用可明显降低牙周炎大鼠牙龈组织TNF-α和IL-6含量,其疗效优于单用大黄素或黄芩苷。

Objective To observe the effects of emodin in combination with baicalin on inflammatory factor of gingival tissue in rats with periodontitis. Methods Periodontitis models in SD rats were prepared with ligation method and high-carbonhydrate diet. They were randomly divided into blank control group, model group, tinidazole group, baicalin group, emodin group, and emodin together with baicalin group. Rats were sacrificed in the 4th week and 8th week separately. Homogenate of gingival tissues was collected from each group to determine contents of TNF-a,IL-6 with ELISA method. Results After building models in rats, their periodontitis symptoms were obvious. Contents of TNF-a ,IL-6 in tissues rose to different degrees, which was most obvious in the model group. Levels of TNF-a, IL-6 in gingival tissues in combination group were significantly lower than those in emodin group（ P 〈 0.05 ） in the 4th week and 8th week. In the 4th week, the levels of TNF-a, IL-6 in combination group were significantly lower than those in baicalin group （P 〈 0.05 ）, while there was no difference between the two groups in the 8th week. Conclusions Combined application of emodin and baicalin can remarkably reduce the contents of TNF-a, IL-6 in gingival tissue in rats with periodontitis, which is better than single application of emodin or baicalin.