滇西亚种树鼩血液学生理指标的测定及其初步分析

Measurement and Analysis of the Hematology Related Physiological Indicators in Tupaia Belangeri Chinensis

目的为建立和评价树鼩-人类疾病模型奠定血液生理学基础.方法滇西亚种树鼩92只,雌雄各半.禁食12 h后,非麻醉状态下心脏采血装入EDTA-K2抗凝管混匀后采用全自动血细胞分析仪进行血液学各项生理指标测定.用SPSS统计软件对测定的数据统计分析并与人的血液学生理指标参考值进行了比较.结果滇西亚种树鼩雌性和雄性血液学生理指标测定值中红细胞数、血红蛋白、红细胞压积、平均血红蛋白浓度、红细胞分布宽度变异系数有显著性差异(P<0.05),其余的指标无显著性差异(P>0.05).滇西亚种树鼩雌雄合并、雌性和雄性血液学生理指标测定值与人的血液学生理指标参考值比较得出:白细胞、中性粒细胞百分比、嗜酸性粒细胞百分比、中性粒细胞绝对值、单核细胞绝对值、红细胞平均体积、平均红细胞血红蛋白含量、平均血红蛋白浓度、红细胞分布宽度均低于人的参考值.淋巴细胞百分比、红细胞数、血红蛋白、红细胞压积、红细胞分布宽度变异系数、血小板数均高于人的参考值.单核细胞百分比、嗜碱性粒细胞百分比、淋巴细胞绝对值、嗜碱性粒细胞绝对值均在人的参考值范围之内.结论测定了滇西亚种树鼩血液学各项生理指标.

Objective To lay the hematdogical physiology foundation for establishment and evaluation of Tu- paia belangeri chinensis model of human disease. Methods We selected 92 Tupaia belangeri ehinensises, in- eluding 46 male and 46 female. After fasting for 12 hours, we collected Tupaia belangeri chinensises＇heart blood in- eluding anesthesia, in EDTA-K2 anticoagulation tube. Then by automatic blood cell analyzer we detected various hematological indexes, and using SPSS statistics software to analyse the data. Finally, the measurement value of Tupaia belangeri chinensis in hematology physiological indicators and the human hematology physiological indicators reference value were compared. Results Tupaia belangeri chinensis in female and male hematology physiology in- dex value had significant difference （P 〈0.05） , such as red blood cell count, hemoglobin, hematocrit, hemoglobin concentration, red cell distribution width coefficient of variation, and the rest of the index value had no significant differences （P〉0.05）. Tupaia belangeri chinensis merge, female and male hematology physiology in- dex value and the human hematology physiological index reference values were compared, and the result showed that the white blood cell, neutrophil, eosinophil percentage percentage, absolute value of neutrophilic granulocyte, monocyte, absolute value of mean corpuscular volume, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration, erythrocyte hemoglobin distribution width are lower than the reference value, and the percentage of lymphocytes, red blood cell count, hemoglobin, hematocrit, red cell distribution width coefficient of variation, platelet counts were higher than the reference value, and mononuclear cell percentage, basophil percentage, abso- lute value of lymphocytes, basophils absolute values were in the human reference value range. Conclusion The hematology physiolozical indicators of tuDaia belangeri chinensis are measured