伊班膦酸钠对成骨细胞分泌骨保护素的调节

Ibandronate controls osteoprotegerin release from osteoblasts

背景:在正畸治疗期间,如何提高成骨速度对缩短正畸时间有很大的帮助。骨保护素能间接抑制破骨细胞成熟,加速牙周骨组织形成。目的:观察分析局部注射伊班膦酸钠对正畸保持阶段牙周组织的影响。方法:选取8周龄雄性SD大鼠55只,随机分为实验组、对照组、空白组。均以50g力牵引上颌右侧第一磨牙近中移动,加力21d。空白组牵引后麻醉处死,实验组和对照组分别于保持前1d局部注射伊班膦酸钠和生理盐水后进入保持期,分别于保持开始后1,3,7,14,21d,每组取5只大鼠麻醉后处死。组织经苏木精-伊红染色,免疫组织化学染色后观察。结果与结论:苏木精-伊红染色观察见实验组破骨细胞少于对照组,相同时间的实验组与对照组比较,实验组骨保护素表达量高于对照组;实验组与对照组中,第1天与第3天骨保护素的表达量无显著性差异(P>0.05),第7,14,21天骨保护素的表达量差异有显著性(P<0.05)。在表达强度上,实验组骨保护素的表达呈逐渐增强的趋势,实验组与对照组骨保护素的表达均强于空白组(P<0.05)。局部注射伊班膦酸钠能使成骨细胞分泌骨保护素增多,间接抑制破骨细胞生成,从而加速骨组织修复形成。

BACKGROUND： During orthodontic treatment, how to the increase osteogenic speed has a beneficia effect to shorten orthodontic time. Osteoprotegerin can indirectly inhibit osteoclast maturation, thereby accelerating periodontal tissue formation. OBJECTIVE： To examine.the effect of local injection of ibandronate on the periodontal tissues during orthodontic process. METHODS： Fifty-five 8-week-old male Sprague-Dawley rats were divided randomly into a blank control group, control group and experimental group. Maxillary first molar （right side） was drawn to mesialmovement under 50 g forces in all rats, lasting 21 days. Rats in the blank group were directly executed anesthetically without any preparation. Rats in the experiment and control groups were locally administrated with ibandronate and normal saline 1 day before retention, and then the retainers were placed. Five rats from the experimental and control groups were executed at 1, 3, 7, 14, 21 days, respectively. Tissue samples from all groups were cut and stained with hematoxylin-eosin and immunohistorchemistry staining. RESULTS AND CONCLUSION： Hematoxylin-eosin staining showed the osteoclasts in the experimental group decreased as compared with the control group. At the same point, osteoprotegerin expressed higher in the experimental group than the control group. However, there was no significant difference in osteoprotegerin level between the experimental group and control group at days 1 and 3 （P 〉 0.05）; a significant difference was found between the two groups at days 7, 14 and 21 （P 〈 0.05）. Expression of osteoprotegerin exhibited an increasing trend in the experimental group. As compared with the blank control group, the expression of osteoprotegerin was significantly higher in the experimenta! and control groups （P 〈 0,05）. Local injection of ibandronate can stimulate osteoblasts to secrete osteoprotegerin and indirectly restrain osteoclast formation, thereby accelerating bone reconstruction.