心肌细胞的三维培养

Three-dimensional culture of cardiomyocytes

背景:由于三维培养可以再现组织细胞的体内生长情况,它已经成为研究肿瘤的耐药性、侵袭性和肿瘤微环境的重要平台。目的:总结归纳了心肌细胞三维培养的研究进展及存在的问题。方法:应用计算机检索CNKI和Pubmed数据库中1994年1月至2012年1月关于心肌细胞三维培养的文章,在标题和摘要中以"三维培养,心肌细胞"或"Three-dimensional culture,Cardiaccell"为检索词进行检索。选择文章内容与心肌细胞三维培养有关者,同一领域文献则选择近期发表或发表在权威杂志文章。初检得到828篇文献,根据纳入标准选择关于心肌细胞三维培养的36篇文献进行综述。结果与结论:寻找满足要求和易于操作的种子细胞是心肌细胞三维培养的关键环节,而种子细胞的空间立体生长要求有支架的依附,支架使种子细胞间形成适宜的空间分布和细胞联系,并可以提供信号诱导细胞的生长和分化。心肌细胞三维培养为心肌修复提供了一个新的思路,虽然此方面研究已取得了很大进展,但仍有很多问题亟待解决,使三维培养的心肌组织血管化是今后研究的主要方向。

BACKGROUND： Three-dimensional culture can reproduce the in vivo growth of tissue cells, and it has become an important platform to study tumor drug resistance, invasion and the tumor microenvironment OBJECTIVE： To summarize the experimental studies about three-dimensional culture of cardiomyocytes and the existing problems. METHODS： CNKI and PubMed （January 1994 to January 2012）were searched for the related articles about three-dimensional culture of cardiomyocytes using the keywords of ＂three-dimensional culture, cardiac cell＂ in Chinese and English, respectively. There were 828 articles after the initial survey, and 36 articles related to three-dimensional culture of cardiomyocytes were included in result analysis. RESULTS AND CONCLUSION： Looking for an ideal seed cell meeting the requirements and easy to operate is the key of the three-dimensional culture of cardiomyocytes, while the spatial growth of the seed cells require a scaffold to be adhered. The scaffold ensures the appropriate spatial distribution and cellcontact among the seed cells, and can provide the signal to induce cell growth and differentiation. Three-dimensional culture of cardiomyocytes provides a new approach for myocardial repair. Although three-dimensional culture of cardiomyocytes in myocardial tissue engineering has made gratifying achievements there are still many problems to be solved, among which, the vascularization of myocardial tissue is the focus of researchers.