纳米TiO_2:C薄膜涂层的生物相容性评价

Biocompatibility of nano-TiO_2:C thin film coating

背景:前期实验采用溶胶-凝胶法在纯钛片表面制备了纳米TiO2:C薄膜涂层。目的:评估纳米TiO2:C薄膜涂层的生物相容性。方法:①溶血实验:取新西兰大白兔静脉血,分别加到TiO2-生理盐水、TiO2:C-生理盐水、蒸馏水和生理盐水中。②细胞毒性实验:将TiO2:C浸提液加入对数生长期的L929细胞培养基中。③短期全身毒性实验:将60只BALB/C小白鼠随机分为3组,分别灌胃给予TiO2浸提液、TiO2:C浸提液及生理盐水。④口腔黏膜刺激实验:将牙胶圆片与TiO2:C纳米薄膜涂片分别缝合固定于仓地鼠颊黏膜上。结果与结论:纳米TiO2:C涂层未引起急性溶血,毒性等级为0-1级,未对细胞产生明显毒性,细胞周期未见明显异常;TiO2:C浸提液对小鼠无短期全身毒性;对仓鼠口腔黏膜无刺激性。表明纳米TiO2:C薄膜涂层具有良好的生物安全性。

BACKGROUND： In previous experiments, TiO2：C thin film coating have been prepared on the surface of titanium paper by sol-gel process.OBJECTIVE： To evaluate the biocompatibility of nanometer TiO2：C thin film coating.METHODS： Hemolysis test： New Zealand white rabbit venous blood was extracted and added into the TiO2-normal saline, TiO2：C-normal saline, distilled water and fresh normal saline. Cytotoxicity test： TiO2：C leaching liquor was added into the L929 cell culture medium in the logarithmic growth phase. Short-term systemic toxicity test： 60 BALB/C mice were randomly divided into three groups and lavaged with leachingliquor extracted from TiO2, TiO2：C coatings and normal saline. Oral mucosa stimulation test： the gutta-percha wafer and the nano-TiO2：C thin film coating were sutured and fixed to the cheek pouch of inaurate hamster. RESULTS AND CONCLUSION： Acute hemolysis was not induced by nano-TiO2：C thin film coating. Toxicity level of nano-TiO2：C thin film coating was 0-1. It had no obvious toxicity to the cells. There was no obvious abnormality of cell cycle. TiO2：C leaching liquor had no short-term systemic toxicity on mice and no stimulation to oral mucosa of hamster. The results showed that nano-TiO2：C thin film coatings have good bio-security.