阳极氧化种植体植入早期骨组织细胞趋化因子受体4的表达

Chemokin CXC motif receptor 4 expression in bone tissue surrounding anode-oxidized implants during early implantation

背景:与传统种植体相比,阳极氧化种植体植入早期即可达到种植稳定,其相关的分子生物学机制并不明确。目的:分析细胞趋化因子受体4在阳极氧化种植体植入早期表达的意义。方法:选取4月龄Wistar大鼠40只,随机分为种植体植入6,12,24h组及空白对照组。将阳极氧化和喷砂两种表面特性的种植体分别植入实验组所有大鼠左右侧胫骨。植入6,12,24h后处死各实验组大鼠,旋出种植体,截取种植体周围直径约2cm骨组织,对照组截取胫骨近骺端直径约2cm骨组织。将各实验组一半骨组织制备蛋白样品,用Westernblot免疫印迹分析细胞趋化因子受体4蛋白,以β-actin作为内参。另一半骨组织进行中性EDTA脱钙、切片,进行细胞趋化因子受体4蛋白免疫组织化学检测。将24h组取出的种植体行扫描电镜观察。结果与结论:Westernblot结果显示,种植体植入24h内,实验组种植体周围骨组织细胞趋化因子受体4蛋白表达量均高于空白对照组。同一时间点,阳极氧化种植体周围骨组织细胞趋化因子受体4表达量均高于表面喷砂种植体周围骨组织。免疫组织化学结果支持上述结果。扫描电镜可见,阳极氧化种植体较喷砂种植体表面黏附较多的间叶样细胞。提示阳极氧化种植体诱导机体骨组织细胞趋化因子受体4高表达,可能促使种植体达到早期稳定。

BACKGROUND： The anode-oxidized implants have shown high implant stability at early period compared to traditional implants. However, the cellular and molecular mechanisms are unknown. OBJECTIVE： To investigate the significance of chemokin CXC motif receptor 4 in bone tissue around anode-oxidized implant during early implantation. METHODS： Forty Wistar rats of 4 months old were selected and randomly divided into 6, 12 and 24 hours implantation groups and blank control group. Rats in experimental groups received anode-oxidized implant in one tibia and sand blast implant in the other one. Rats in the experimental groups were killed at 6, 12 and 24 hours accordingly after implantation, the implants were unscrewed, and the bone tissue around implants was prepared, 2 cm in diameter. For blank control group, proximal epiphysis bone tissue about 2 cm in diameter were prepared. Half bone tissues in the experimental groups were made into protein samples. The expression of chemokin CXC motif receptor 4 was detected by Western blot with 13-actin as internal reference. The other half bone tissues were treated with neutral ethylene diamine tetraacetie acid, and then slides were made. The expression of chemokin CXC motif receptor 4 was detected by immunohistochemistry. The implants in the 24 hours implantation group were examined by scanning electron microscope. RESULTS AND CONCLUSION： The results of Western blot demonstrated that the expression of chemokin CXC motif receptor 4 in rat bone tissue of the experimental groups were significantly higher than that of the blank control group within 24 hours after implantation. At the same time point, the expression of chemokin CXC motif receptor 4 in rat bone tissue around the anode-oxidized implant was higher than that around the sand blast implant. The results of immunohistochemistry were consistent with those of Western blot. The observation results of scanning electron microscope showed that anode-oxidized surface showed more attached mesenchymal-like calls scattered all over the surfaces than the sand blast implant. The anode-oxidized implants can induce higher expression of chemokin CXC motif receptor 4 around bone tissue, and may promote the stability of implant at early time points.