同时性多中心尿路上皮癌克隆起源的FISH研究

The clonal origin of multifocal urothelial carcinoma studied with fluorescence in situ hybridization

目的探讨同时性多中心尿路上皮癌的克隆起源。方法通过荧光原位杂交(fluorescence in situ hybridization,FISH)技术对20例病例,49枚肿瘤的3、7、9、17号染色体的变异类型进行检测,判断肿瘤多中心灶间染色体变异类型是否一致。结果 12例多中心灶各瘤灶间CSP3、CSP7、CSP17及GLP p16变异类型完全相同;8例GLP p16改变相同而CSP3、CSP7、CSP17变异类型不完全一致,其中6例GLP p16为相同的缺失改变,2例GLP p16为相同的非缺失改变。结论 20例病例中,18例(90%)有相同克隆起源,另外2例亦不能排除为同一克隆起源可能,提示绝大多数同时性多中心尿路上皮癌为单克隆起源。

Objective To investigate the clonal origin of multifocal urothelial carcinoma.Methods The aberration types of chromosomes 3,7,9 and 17 in 20 cases of multifocal urothelial carcinoma with a total of 49 tumors were detected with fluorescence in situ hybridization（FISH）.Results 12 cases had the same type of 3,7,9 and 17 chromosomal aberrations.8 cases had the same type of 9 chromosomal aberrations and different types of 3,7,and 17 chromosomal aberrations,6 of which displayed loss of chromosomal 9p21,and 2 cases non-loss of chromosomal 9p21.Conclusions Most simultaneous multifocal urothelial carcinomas are mostly of monoclonal origin.