奶白菜核基因雄性不育系的选育与利用

Breeding and Utilization of the Genetic Male Sterile Line in Milk Chinese Cabbage

为解决奶白菜杂交种生产中的杂交制种手段问题,配制优良杂交种。以核不育"复等位基因遗传"假说为指导,以青梗白菜核基因雄性不育系00S107作不育源,采用连续回交转育同时测交鉴定基因型的方法,定向转育奶白菜核基因雄性不育系。并利用转育成的不育系与奶白菜优良自交系配制杂交组合,进行了杂种优势分析。选育出园艺学性状与目标品系相似,具有100%不育株率和不育度的奶白菜核基因雄性不育系GMS3,筛选出2个产量高、整齐度高的优异杂交组合GMS3×B1、GMS3×B2。定向转育模式兼顾了不育基因和园艺学性状的转育,解决了奶白菜核不育系转育和利用的难题。

In order to resolve the problem that cross breeding and production of hybrid in milk Chinese cabbage.According to the hypothesis of multiple allele inherited genetic male sterile,A genetic male sterile line of Chinese cabbage with green stipe 00S107 was used as a source of male sterility,and methods of continuous backcrossing and identifying of the genotypes were applied to transfer the male sterile line of milk Chinese cabbage.Hybridized combinations were obtained by the male sterile line as female parent cross with excellent self-line and we analysed the hybrid vigor.A new genetic male sterile line GMS3 with 100% male sterile plants and 100% male sterility was obtained.Two excellent hybridized combinations GMS3×B1,GMS3×B2 were selected,which is uniform and superior to CK in yield.Directional transfer model which transfer sterile gene and horticultural characters at the same time resolved the puzzle that breeding and utilization of the genetic male sterile line in milk Chinese cabbage.