冬小麦叶片对低温胁迫的生理响应

Physiological Responses of Winter Wheat′s Leaves to Low Temperature Stress

以抗寒性不同的冬小麦品种(米808、冬引0801、小黑麦、冬麦9625、冬麦138)为试验材料,测定各材料在不同温度(13,7,4,-18,-21℃)下处理2 h后叶片的相对电导率、叶片中丙二醛(MDA)、可溶性蛋白质、脯氨酸、可溶性糖含量和超氧化歧化酶的活性(SOD)。结果表明,随着温度降低(4℃→-18℃→-21℃),小麦叶片中MDA、可溶性糖含量逐渐增加。小麦叶片SOD总活力随胁迫温度降低先升高后降低再升高。经低温胁迫后,小麦叶片可溶性蛋白质含量无明显变化。米808、冬引0801在-21℃胁迫下,相对电导率变化率最低。米808、冬引0801在-18℃→-21℃时,MDA含量增幅较大;米808 SOD总活力在-18、-21℃时变化率最低。冬引0801脯氨酸和可溶性糖的变化率均在21℃时最大。

Different cool-resistance varieties were used to experimental material to test relative conductivity,malondialdehyde,soluble protein,proline,soluble sugar content and the activities of superoxide dismutase after 2 hours treatment with 13,7,4,-18,-21 ℃.As the temperature was reduced,MDA and soluble sugar content of wheat leaf increase gradually,the activities of SOD trend is N-shaped wave.After low temperature stress,the soluble protein content of wheat leaves no significant change.The results indicated that M808 and Dongyin0801 have the minimum relative electric conductivity in 21 ℃.MDA content of M808 and Dongyin0801 increased considerably from-18 ℃ to-21 ℃.M808′s activities of SOD has the lowest rate of change in-18 ℃ and-21 ℃.Proline and soluble sugar content of Dongyin0801 has the largest rate of change in-21 ℃.