HPV6/HPV11型病毒在儿童复发性呼吸道乳头状瘤中的表达

Expression of HPV6/HPV11 in juvenile onset recurrent respiratory papillomatosis

目的研究儿童复发性呼吸道乳头状瘤(juvenile onset recurrent respiratory papillomatosis,JORRP)的患儿首次发病及完全治愈时肿瘤组织中HPV6型和HPV11型病毒含量的表达变化以及其病毒感染率。方法收集从首次发病到完全治愈的JORRP患儿29例,分别取每例患儿首次发病及治愈时的标本,采用qRT-PCR法分别测定其首次发病及完全治愈时乳头状瘤组织标本中HPV病毒的含量。结果①首次发病组与治愈组HPV6型与HPV11型病毒qRT-PCR相对定量结果的差异均无统计学意义(P>0.05)。②首次发病组与治愈组HPV6型病毒感染的总体阳性率相同(P>0.05);HPV11型病毒感染的总体阳性率不同(P<0.05)。结论①完全治愈组与首次发病组的JORRP患儿相比,HPV6型与HPV11型病毒量的表达并无明显变化。②首次发病组的患儿均有HPV11型病毒感染,治愈组的患儿HPV11型病毒感染率有所下降。

Objective To investigate the expression of human papilloma virus 6 （HPV6） and HPV 11 in juvenile onset recurrent respira- tory papilloma（JORRP） and examine differences in HPV load and infection rate at initial infection and after complete cure. Methods A total of 29 children with JORRP were analyzed by real-time fluorescent quantitative PCR to determine HPV load at initial infection and after complete cure. Results （~） HPV6 and HPVll load did not differ significantly from each other at initial infection or after complete cure （P 〉 0.05 ）.（~） The rate of HPV6 infection was similar at initial infection and after complete cure（P 〉 0.05 ） ,while the overall rate of HPV11 infection was different （P 〈 0.05 ）. Conclusion HPV6 and HPV11 load did not differ significantly from each other at initial infec- tion or after complete cure. Children had HPV11 virus at initial infection, and the rate of HPV11 infection declined after complete cure.