班氏促卵助孕煎剂含药血清对体外培养人卵巢颗粒细胞增殖的影响

Effect of Serum Containing Banshi Culuan Zhuyun Soup on Proliferation of Ovarian Granulosa Cells of Human

目的运用血清药理学方法观察班氏促卵助孕煎剂对人卵巢颗粒细胞增殖的影响。方法向预培养24 h的人卵巢颗粒细胞中分别加入10%空白血清(对照组)、班氏促卵助孕煎剂含药血清(高、中、低剂量组),应用噻唑蓝(MTT)法分别检测0,12,24,36,48,60,72 h的颗粒细胞增殖情况。结果 24 h前班氏促卵助孕煎剂对细胞增殖的作用不大(P>0.05);36,48,60和72 h显示低、中、高三组班氏促卵助孕煎剂含药血清吸光度均明显高于空白血清组,差异有统计学意义(P<0.01),与低浓度组相比,高、中浓度组均差异有统计学意义(P<0.01),但高浓度组和中浓度组之间差异无统计学意义(P>0.05)。结论班氏促卵助孕煎剂含药血清能明显促进体外培养人卵巢颗粒细胞的增殖,且呈剂量相关性。

Objective To observe the effect of serum containing bartshi culuan zhuyun soup on proliferation of human ovarian granulsa cells. Methods According to the serum pharmacological method, serum containing high, medium and low doses of banshi culuan zhuyun soup was prepared. The human ovarian granulosa cells were collected from the patients and incubated for 24 h. The serum containing three different doses of banshi culuan zhuyun soup and normal serum were added to the cell culture system. MTT assay was used to detect the cell proliferation at 0, 12, 24, 36, 48, 60, and 72 h. Results MTr assay showed that the serum containing banshi culuan zhuyun soup did not have significant effect on cell proliferation before 24 h （P〉0.05）. But at 36, 48, and 72 h, it showed that the A values of the different experimental groups were significantly higher than that of the control group （P〈0.01）. Compared with the low dose group, A values of the medium and high dose groups were significantly .different （P〈0.01）. There was no significant difference in A values between the high dose and medium dose groups （P〉0.05）. Conclusion Serum containing banshi culuan zhuyun soup can effectively promote proliferation of the ovarian granulosa cells in a dose-dependent manner.