高效液相色谱-蒸发光散射法测定铁皮石斛中单糖和双糖

Determination of Monosaccarides and Dissaccharides in Dendrobium officinale by HPLCELSD

目的建立高效液相色谱-蒸发光散射(HPLC-ELSD)测定铁皮石斛药材中单糖和双糖的含量分析方法。方法色谱柱Shodex Asahipak NH2(250 mm×4.6 mm,5μm),流动相为乙腈-水(78∶22),流速1.0 mL.min-1,柱温30℃;ELSD参数:漂移管温度为100℃,气体流量为1.60 L.min-1。结果果糖、葡萄糖和蔗糖分别在1.38～7.69μg(r=0.999 8),4.87～23.31μg(r=0.999 9),5.91～36.28μg(r=0.999 9)范围内呈良好的线性关系,加样回收率在95.20%～103.0%之间。结论该方法简便、准确、重复性好,适用于铁皮石斛中单糖和双糖的含量测定。

Objective To develop a method for determination of monosaccarides and dissaccharides in Dendrobium officinale by HPLC-ELSD. Methods Shodex Asahipak NH2（250 mm×4.6 mm, 5 μm） was used as chromatographic column. Mobile phrase was acetonitrile-water （78 ： 22）. Flow rate was set at 1.0 mL · min^-1. Column temperature was 30 ℃ . ELSD parameters were as follows： temperature of drift tube： 100 ℃, gas flow： 1.60 L · min^-1. Results The assay displayed good linearity. The linear ranges were from 5.91 to 36.28 μg （ r = 0. 999 8 ） for fructose, from 4.87 to 23.31 μg （ r = 0. 999 9） for glucose, and from 5.91 to 36.28 txg （ r=0.999 9） for sucrose. The recoveries ranged from 95.20% to 103.0%. Conclusion The method possesses advantages of easy operation, high accuracy and good reproducibility, and is suitable for determination of monosaccarides and dissaccharides in Dendrobium officinale.