雨生红球藻光合和呼吸速率研究

PHOTOSYNTHETIC AND RESPIRATION RATE OF HAEMATOCOCCUS PLUVIALIS

于 1 994年在青岛以单细胞雨生红球藻为实验材料 ,利用MCM改良配方制作培养液 ,采用测溶解氧的方法 ,进行了光合和呼吸速率测定。结果表明 ,雨生红球藻光合速率、呼吸速率不仅与温度、光照强度有密切关系 ,同时还与其生活史中不同发育阶段有关。 1 7℃、2 5℃、33℃时 ,雨生红球藻游动细胞光饱和点光强分别为 2 60 μE/ (m2 ·s)、32 0 μE/ (m2 ·s)、32 0 μE/ (m2 ·s)左右 ,光补偿点光强分别为 80 μE/ (m2 ·s)、70 μE/ (m2 ·s)、1 0 0 μE/ (m2 ·s)左右。2 5℃时 ,不动细胞的光合和呼吸速率均明显低于游细胞 ,其光补偿点和光饱和点光强均比游细胞的高 ,分别为 90 μE/ (m2 ·s)、360 μE/ (m2 ·s)左右。

Photosynthetic and respiration rate of Haematococcus pluvialis cultured in modified MCM medium under artificial conditions was calculated by measuring the rate of oxygen production and consumption in 1994 in Qingdao. The results show that 1) both photosynthetic rate and respiration rate are typically associated with light intensity and temperature; 2) light intensity at photosynthetic saturated point of motile cells is 260, 320, 320μE/(m 2·s),respectively when cultured at 17℃, 25℃ and 33℃, meanwhile, light intensity at photosynthetic compensation points is 80, 70, 100μE/(m 2·s); and 3) photosynthetic capacity increases with light intensity until light saturated point,and then decreases with further increase of illumination which is called photoinhibition. The results also indicate that cell development stages of this unicellular alga play an important role in photosynthetic and respiration rate, that light intensities at compensation point and saturated point of non motile cells are 90 and 360μE/(m 2·s),both higher than those of motile cells, whereas both photosynthetic capacity and respiration rate of non-motile cells are lower than those of motile cells. Based on the above results and exact natural environment discussed, radiation for each cell ranging from 200 to 320μE/(m 2·s) and large temperature difference between day and night are expected by various means of regulation such as cell density, culture depth, mixing and so on, and are valuable for the cultivation of this algae.