原发性ITP患儿外周血血小板相关抗体和淋巴细胞亚群的变化及临床意义

The Changes and Clinical Significance of Platelet Antibodies and Lymphocyte Subsets in Peripheral Blood of Children with Primary ITP

目的:观察原发性ITP患儿外周血血小板抗体及淋巴细胞亚群变化,探讨其与ITP的关系及临床意义。方法:采用流式细胞技术检测患儿与正常儿童外周血血小板相关抗体(PAIgA、G、M)水平及淋巴细胞亚群(CD3+、CD3+CD4+、CD3+CD8+、CD4+/CD8+、CD19+、CD3-CD(16+56)+)水平。结果:新诊断ITP组与持续性ITP组PAIg(G、M)水平明显高于正常儿童(P<0.001);新诊断性ITP组与持续性ITP组和对照组比较,CD3+T细胞、CD4+/CD8+T细胞比值、CD3-CD(16+56)+NK细胞降低,CD3+CD8+T细胞、CD19+B细胞升高,差异均有统计学意义(P<0.05)。结论:原发性ITP患儿存在细胞免疫和体液免疫紊乱,血小板相关抗体和淋巴细胞亚群水平检测的联合使用对原发性ITP的诊断、疗效评估有较好的实用价值。

@@@@Objective:To observe the changes of platelet antibodies and lymphocyte subsets in peripheral blood of children with ITP. To investigate the relationship between the changes and ITP,as well as clinical significance. Method:The flow cytometry was used to detect the level of platelet antibody (PAIgA,G,M)and lymphocyte subsets(CD3+、CD3+CD4+,CD3+CD8+,CD4+/CD8+,CD19+,CD3-CD(16+56)+)in peripheral blood of children with primary ITP and healthy children. Result:Newly diagnosed and persistent ITP patients’ platelet antibodies(PAIgA,G,M)was significantly higher than that of normal children(P<0.001). Newly diagnosed and persistent ITP patients’ CD3+T cell,CD4+/CD8+T cell,CD3-CD(16+56)+NK cells was lower than that of normal children. CD3+CD8+T cell、CD19+B cell was higher and the difference was statistically significant(P<0.05). Conclusion:Children with primary ITP existed cell immunity and humoral immune disorders. Platelet antibodies combined with lymphocyte subsets has good practical value to the diagnosis and curative effect evaluation of primary ITP.