白蚁mtDNA提取方法改良及检测

Improvement and Check on the mtDNA extract techniques for termites

利用mtDNA多态性进行种类鉴定是一种分子生物学常用方法,从DNA水平对白蚁进行物种鉴别并探讨物种的进化,其必要前提是提取到一定数量和质量的mtDNA.在分离得到线粒体后,分别采用CTAB、SDS2种方法提取mtDNA.紫外分光光度计检测DNA纯度及浓度,用mtDNA特异性引物进行PCR扩增检测.试验证明2种方法均能成功提取白蚁的mtDNA,SDS法提取效果较好.

Species identification with mtDNA polymorphisms is an usual method in molecular biology. To identify the species and to explore the evolution of the termites from the DNA level, it is necessary to extract the mtDNA with enough quantity and good quality. In this study, mitochondria of termites is firstly isolated, then CTAB and SDS methods were employed for the extraction of mtDNA, respectively. Purity and concentration of mtDNA were determined by UV spectrophotometer. PCR amplification of mtDNA was executed by specific primers. Results shows that both CTAB the SDS methods can successfully extracted termite mtDNA, but SDS method shows better performance than CTAB method.