阿魏酸和香豆素对铜绿微囊藻的化感作用

Allelopathy Effects of Ferulic Acid and Coumarin on Microcystis aeruginosa

通过对铜绿微囊藻(Microcystis aeruginosa)的藻液D680、叶绿素a、电导率(EC)值以及超氧阴离子自由基O.2-含量的测定,研究了不同浓度的阿魏酸和香豆素对铜绿微囊藻的生长抑制作用及其机制.结果表明,阿魏酸和香豆素对铜绿微囊藻的生长均产生"低促高抑"作用,浓度高于100 mg.L-1的阿魏酸和香豆素对铜绿微囊藻表现出明显的抑制作用,200 mg.L-1的阿魏酸和香豆素第6 d的平均抑藻率分别为80.3%和58.0%.在高浓度阿魏酸和香豆素抑制作用下,铜绿微囊藻的叶绿素a含量迅速降低、EC值和O.2-含量明显增大,说明化感物质可能通过破坏细胞膜、增加O.2-含量、降低叶绿素a含量等作用抑制藻细胞的生长.此外,种子发芽实验结果表明,阿魏酸较香豆素毒性小.

The inhibitory effects and allelopathy mechanism of ferulic acid and coumarin on Microcystis aeruginosa were investigated by measuring the D680 value,the content of chlorophyll-a,the electrical conductivity（EC） and superoxide anion radical O·-2 value.Ferulic acid and coumarin had allelopathic effects on the growth of M.aeruginosa and promoted the physiological metabolism at low concentrations while inhibited the metabolism at high concentrations.Obvious inhibitory effects were observed when the concentration of ferulic acid or coumarin was over 100 mg·L-1.The average inhibitory rates reached 80.3% and 58.0% after six days when the concentration of ferulic acid or coumarin was 200 mg·L-1.The content of chlorophyll-a was decreased while the EC value and O·-2 concentration were promoted by higher concentrations of ferulic acid or coumarin,suggesting that the growth of algae was inhibited probably by the damage of cell membrane,increase in the content of O·-2 and decrease in the content of chlorophyll-a.In addition,seed germination test elucidated that Ferulic acid was safer than Coumarin.