行气润肠汤治疗便秘型肠易激综合征的实验研究

Experimental Study on Irritable Bowel Syndrome of Constipation Type Treated with Xingqi Runchang Decoction

目的:探讨行气润肠汤对便秘型肠易激综合征大鼠5-羟色胺(5-HT)、P物质(SP)、血管活性肽(VIP)的影响。方法:采用结直肠球囊扩张刺激及限水法建立便秘型IBS大鼠模型,实验设正常组、模型组、行气润肠汤组、福松组,造模成功后第2天起,行气润肠汤组、福松组分别给予中药溶液、福松溶液2 mL灌胃,正常组、模型组以生理盐水等容量灌胃。每组均连续灌胃14 d。灌胃期间各组大鼠总饮水量均控制在200 mL.d-1。实验结束后测结肠组织5-HT、SP、VIP的含量,并比较各组差异。结果:模型组大鼠结肠5-HT、SP、VIP含量呈上升趋势,与正常组比较,有显著差异(P<0.01)。与模型组比较,行气润肠汤组5-HT、SP、VIP含量明显降低(P<0.01)。结论:行气润肠汤能降低便秘型肠易激综合征大鼠肠道5-HT、VIP、SP的分泌,从而改善便秘症状。

Objective： To investigate the effect of Xingqi Runchang Decoction on 5-serotonin（5-HT）,substance P（SP）,vasoactive intestinal peptide（VIP） of rats with irritable bowel syndrome of constipation type.Methods： IBS model rats of constipation type were established with the method of colorectal balloon dilatation stimulation and limit,the experimental rats were divided into normal control group,model group,Xingqi Runchang Decoction,Fukumatsu group.The second day after the modeling,Xingqi Runchang Decoction and Fukumatsu group were respectively given gastric perfusion of 2 mL Chinese medicinal solution and Fukumatsu solution,while the normal group and model group were respectively given gastric perfusion of 2 mL saline.Each group was given gastric perfusion for 14 D.During the gastric perfusion,the total amount of drinking water in each group was controlled at 200 mL·d-1.The content of colon tissue 5-HT,SP,VIP was measured after the experiment,and their difference was compared.Results： Colon 5-HT,SP,VIP content increased in the model group,there was significant difference（P＆lt;0.01）,compared with the normal group.Compared with the model group,Xingqi Runchang Decoction group,5-HT,SP,VIP content decreased significantly（P＆lt;0.01）.Conclusion： Xingqi Runchang Decoction can reduce the intestinal synthesis secretion of 5-HT,VIP,SP of rats with irritable bowel syndrome of constipation type,thereby improving the symptoms of constipation.